Center for Reproductive Endocrinology and Infertility, Hue University of Medicine and Pharmacy, Hue University, Vietnam.
Department of Obstetrics and Gynecology, Hue University of Medicine and Pharmacy, Hue University, Vietnam.
J Int Med Res. 2022 May;50(5):3000605221097492. doi: 10.1177/03000605221097492.
This study was performed to determine the effect of swim-up (SU) and density gradient centrifugation (DGC) on sperm survival and DNA fragmentation.
Individual semen samples were analyzed before each was divided into two aliquots (half for SU and half for DGC) for calculation of sperm survival and the DNA fragmentation index (DFI). Sperm DNA fragmentation was determined using the sperm chromatin dispersion test.
The DFI of the 63 semen samples processed using both procedures was lower than that of the fresh semen samples. The DFI was significantly lower for samples processed using the SU than DGC method. In the sperm survival test, the SU technique was associated with increased sperm motility and vitality following preparation. After 24 hours, however, the concentration and percentage of surviving sperm were significantly lower in the SU than DGC group.
Both semen preparation techniques help to minimize sperm DNA fragmentation; however, when the DFI is <30%, the SU technique is more appropriate than DGC. While DGC may be superior for intrauterine insemination, the SU method may be preferable for in vitro fertilization or maturation.
本研究旨在确定泳动（SU）和密度梯度离心（DGC）对精子存活和 DNA 碎片化的影响。
在每个精液样本被分成两份（一半用于 SU，一半用于 DGC）进行精子存活和 DNA 碎片化指数（DFI）计算之前，对单个精液样本进行分析。使用精子染色质扩散试验来确定精子 DNA 碎片化。
用两种方法处理的 63 个精液样本的 DFI 均低于新鲜精液样本。与 DGC 方法相比，SU 处理的样本 DFI 明显更低。在精子存活试验中，SU 技术在准备后与精子活力和活力的增加相关。然而，24 小时后，SU 组的精子浓度和存活率明显低于 DGC 组。
两种精液制备技术都有助于最大限度地减少精子 DNA 碎片化；然而，当 DFI<30%时，SU 技术比 DGC 更合适。虽然 DGC 可能更适合宫腔内人工授精，但 SU 方法可能更适合体外受精或成熟。
