Xue Xia, Wang Wan-Shan, Shi Juan-Zi, Zhang Si-Lin, Zhao Wan-Qiu, Shi Wen-Hao, Guo Bao-Zhu, Qin Zhen
J Assist Reprod Genet. 2014 Sep;31(9):1161-6. doi: 10.1007/s10815-014-0287-z.
To compare the efficacy of swim-up and DGC in improving sperm deformity and DNA fragmentation and to determine which method is better in teratozoospermic patients requiring artificial reproduction.
The present study compared the effects of swim-up and density gradient centrifugation (DGC), the two most commonly used semen preparation methods, on sperm deformity rate and DNA fragmentation index (DFI) in semen samples from teratozoospermic patients.
The results demonstrated that both swim-up and DGC yielded a significantly lower sperm deformity rate and DFI in comparison to unprocessed whole semen, with DGC having more favorable results. Sperm deformity rate in unprocessed whole semen samples was significantly lower in the 20-29 age group than in the 40-49 age group, but no significant difference was observed in DFI between different age groups. There was no significant correlation between sperm deformity rate and DFI.
Our findings suggest that enrichment of sperm with normal morphology and intact DNA in teratozoospermic patients could be achieved by both DGC and swim-up procedures, and that DGC is a better method.
比较上游法和密度梯度离心法（DGC）在改善精子畸形率和DNA碎片化方面的效果，并确定在需要人工生殖的畸形精子症患者中哪种方法更佳。
本研究比较了两种最常用的精液处理方法——上游法和密度梯度离心法（DGC）对畸形精子症患者精液样本中精子畸形率和DNA碎片化指数（DFI）的影响。
结果表明，与未处理的全精液相比，上游法和DGC均能显著降低精子畸形率和DFI，且DGC的效果更佳。未处理的全精液样本中，20-29岁年龄组的精子畸形率显著低于40-49岁年龄组，但不同年龄组之间的DFI无显著差异。精子畸形率与DFI之间无显著相关性。
我们的研究结果表明，DGC和上游法均可使畸形精子症患者获得形态正常且DNA完整的精子，且DGC是更好的方法。
