Department of Pathology, College of Medicine, University of Babylon, Babylon, Iraq.
Al-Noor Pediatric Hospital, Babil Health Directorate, Republic of Iraqi Ministry of Health, Babylon, Iraq.
Arch Razi Inst. 2021 Dec 30;76(6):1639-1643. doi: 10.22092/ari.2021.356358.1825. eCollection 2021 Dec.
Inflammatory bowel disease (ulcerative colitis and Crohn's disease) is a remitting and relapsing disease that affects millions of people worldwide. In the intestine, homeostasis between repair and signaling of the proinflammatory of the immune system is needful for the upkeep of intestinal balance. The recent evidence has highlighted the dysfunction of the immune system, particularly toll-like receptors (TLRs)-mediated immune system dysfunction, in the ulcerative colitis pathogenesis. The main objective of the current study was to analyze the expression level of TLR5 at the mRNA in ulcerative colitis patients and investigate the impacts of the TLR5 gene as genetic factors that contribute to the development of ulcerative colitis. The paraffin-embedded blocks were retrospectively collected from 25 patients diagnosed with ulcerative colitis and 25 cases with normal colonic tissue. The quantitative real-time polymerase chain reaction method was employed for the estimation of TLR5at the mRNA level. The analysis of the data of TLR5 gene expression revealed that the expression of this gene is downregulated in ulcerative colitis cases, as compared to that in normal colonic tissue. As evidenced by the results of this study, TLR5 may play a role in the development of ulcerative colitis; therefore, it should be introduced in the management of ulcerative colitis.
炎症性肠病（溃疡性结肠炎和克罗恩病）是一种反复发作的疾病，影响着全球数百万人。在肠道中，修复和信号转导之间的平衡对于维持肠道平衡是必要的。最近的证据强调了免疫系统的功能障碍，特别是 TLR（Toll 样受体）介导的免疫系统功能障碍，在溃疡性结肠炎发病机制中的作用。本研究的主要目的是分析溃疡性结肠炎患者 TLR5 的 mRNA 表达水平，并探讨 TLR5 基因作为遗传因素对溃疡性结肠炎发病的影响。从 25 例溃疡性结肠炎患者和 25 例正常结肠组织的石蜡包埋块中回顾性收集。采用定量实时聚合酶链反应方法估计 TLR5 的 mRNA 水平。TLR5 基因表达数据的分析表明，与正常结肠组织相比，该基因在溃疡性结肠炎病例中的表达下调。本研究结果表明，TLR5 可能在溃疡性结肠炎的发病机制中起作用；因此，它应该被引入溃疡性结肠炎的治疗中。
