Department of Basic Sciences, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tehran, 14155-6453, Tehran, Iran.
Arch Razi Inst. 2021 Dec 30;76(6):1735-1744. doi: 10.22092/ari.2021.354188.1626. eCollection 2021 Dec.
The present study aimed to assess the probable impact of the central histaminergic and melanocortin systems on leptin-induced hypophagia in neonatal layer chickens. In experiment 1, the chickens received intracerebroventricular (ICV) injections of the control solution, 250 nmol of α-FMH, 10 µg of leptin, and α-FMH+leptin. Experimental groups 2-8 were injected the same as experiment 1. Nonetheless, the chickens in experiments 2-8 received ICV injections of 300 nmol of chlorpheniramine (H receptor antagonist), 82 nmol of famotidine (H receptor antagonist), 300 nmol of thioperamide (H receptor antagonist), 0.5 nmol of SHU9119 (M/M receptors antagonist), 0.5 nmol of MCL0020 (M receptor antagonist), 30 µg of astressin-B (CRF/ CRF receptors antagonist), and 30 µg of astressin2-B (CRF receptor antagonist), instead of α-FMH, respectively. Food was provided for the birds immediately following the injection, and 30, 60, and 120 min after the injection, cumulative food intake (g) was measured. The findings pointed out that the ICV injection of leptin diminished food intake in neonatal chickens (<0.05). The co-administration of M/M receptor antagonist+leptin significantly decreased the hypophagic effect of leptin (<0.05). A significant decrease was also detected in the hypophagic effect of leptin following the co-administration of the M receptor antagonist and leptin (<0.05). Moreover, the co-injection of the antagonists of CRF/CRF receptors and leptin significantly mitigated the hypophagic effect of leptin (<0.05). The co-injection of CRF receptor antagonist and leptin led to a decrease in the hypophagic effect of leptin. As evidenced by the results of the current study the hypophagic effect of leptin is mediated by the receptors of H, H, M/M, and CRF/CRF in neonatal layer chicken.
本研究旨在评估中枢组胺能和黑色素皮质素系统对新生蛋鸡瘦素诱导的摄食减少的可能影响。在实验 1 中，鸡接受了脑室（ICV）注射对照溶液、250 nmol α-FMH、10 µg 瘦素和α-FMH+瘦素。实验组 2-8 接受了与实验组 1 相同的注射。然而，实验组 2-8 的鸡接受了脑室注射 300 nmol 氯苯那敏（H 受体拮抗剂）、82 nmol 法莫替丁（H 受体拮抗剂）、300 nmol 噻哌酰胺（H 受体拮抗剂）、0.5 nmol SHU9119（M/M 受体拮抗剂）、0.5 nmol MCL0020（M 受体拮抗剂）、30 µg astressin-B（CRF/CRF 受体拮抗剂）和 30 µg astressin2-B（CRF 受体拮抗剂），而不是 α-FMH。注射后立即为鸟类提供食物，并在注射后 30、60 和 120 分钟测量累积食物摄入量（g）。研究结果表明，脑室注射瘦素可减少新生鸡的摄食量（<0.05）。M/M 受体拮抗剂+瘦素的共同给药显著降低了瘦素的摄食减少作用（<0.05）。M 受体拮抗剂和瘦素共同给药后，瘦素的摄食减少作用也显著降低（<0.05）。此外，CRF/CRF 受体拮抗剂和瘦素的共同注射显著减轻了瘦素的摄食减少作用（<0.05）。CRF 受体拮抗剂和瘦素的共同注射导致瘦素的摄食减少作用降低。本研究结果表明，瘦素的摄食减少作用是由新生蛋鸡的 H、H、M/M 和 CRF/CRF 受体介导的。
