Wood Jane, van der Gast Christopher, Rivett Damian, Verran Joanna, Redfern James
Manchester Fashion Institute, Faculty of Arts and Humanities, Manchester Metropolitan University, Manchester, United Kingdom.
Department of Life Sciences, Faculty of Science and Engineering, Manchester Metropolitan University, Manchester, United Kingdom.
Front Bioeng Biotechnol. 2022 Apr 25;10:876822. doi: 10.3389/fbioe.2022.876822. eCollection 2022.
The textile industry is in crisis and under pressure to minimize the environmental impact on its practices. Bacterial cellulose (BC), a naturally occurring form of cellulose, displays properties superior to those of its cotton plant counterpart, such as enhanced purity, crystallinity, tensile strength, and water retention and is thus suitable for an array of textile applications. It is synthesized from a variety of microorganisms but is produced in most abundance by . is available as a type strain culture and exists in the microbial consortium commonly known as Kombucha. Whilst existing literature studies have described the effectiveness of both isolates and Kombucha in the production of BC, this study investigated the change in microbial communities across several generations of sub-culturing and the impact of these communities on BC yield. Using Kombucha and the single isolate strain as inocula in Hestrin and Schramm liquid growth media, BC pellicles were propagated. The resulting pellicles and residual liquid media were used to further inoculate fresh liquid media, and this process was repeated over three generations. For each generation, the thickness of the pellicles and their appearance under SEM were recorded. 16S rRNA sequencing was conducted on both pellicles and liquid media samples to assess changes in communities. The results indicated that the genus was the most abundant species in all samples. Cultures seeded with Kombucha yielded thicker cellulose pellicles than those seeded with , but all the pellicles had similar nanofibrillar structures, with a mix of liquid and pellicle inocula producing the best yield of BC after three generations of sub-culturing. Therefore, Kombucha starter cultures produce BC pellicles which are more reproducible across generations than those created from pure isolates of and could provide a reproducible sustainable model for generating textile materials.
纺织业正处于危机之中，面临着将其生产活动对环境的影响降至最低的压力。细菌纤维素（BC）是纤维素的一种天然存在形式，其性能优于棉花，如纯度更高、结晶度更高、拉伸强度更高以及保水性更强，因此适用于一系列纺织应用。它由多种微生物合成，但最主要由 生产。 作为模式菌株培养物可得，存在于通常被称为康普茶的微生物群落中。虽然现有文献研究描述了 分离株和康普茶在生产BC方面的有效性，但本研究调查了几代继代培养过程中微生物群落的变化以及这些群落对BC产量的影响。在赫斯特林和施拉姆液体生长培养基中，以康普茶和单一分离株 作为接种物来繁殖BC菌膜。将得到的菌膜和残留液体培养基用于进一步接种新鲜液体培养基，这个过程重复三代。对于每一代，记录菌膜的厚度及其在扫描电子显微镜下的外观。对菌膜和液体培养基样本进行16S rRNA测序以评估群落变化。结果表明， 在所有样本中是最丰富的物种。用康普茶接种的培养物产生的纤维素菌膜比用 接种的更厚，但所有菌膜都有相似的纳米纤维结构，在三代继代培养后，液体和菌膜接种物混合产生的BC产量最高。因此，康普茶起始培养物产生的BC菌膜在各代之间比由 的纯分离株产生的更具可重复性，并且可以为生产纺织材料提供一个可重复的可持续模型。
