Wang Junhong, Shi Chunwei, Cheng Mingyang, Lu Yiyuan, Zhang Xiaoyu, Li Fengdi, Sun Yu, Li Xiaoxu, Li Xinyang, Zeng Yan, Wang Chunfeng, Cao Xin
College of Veterinary Medicine, Jilin Agricultural University, Changchun, China.
Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Jilin Agricultural University, Changchun, China.
Front Cell Dev Biol. 2022 Apr 25;10:874525. doi: 10.3389/fcell.2022.874525. eCollection 2022.
Zbtb1 (zinc finger and BTB domain containing 1) is a member of mammalian zbtb gene family. A series of bioinformatics analysis was carried out for the EL4 cell and the Zbtb1-deficient EL4 cell by Hi-C, ATAC-seq and RNA-seq techniques. Finally, Hi-C results showed that the intensity of chromatin interaction in the deletion group decreased with distance, the degree of chromosome interaction decreased significantly, the AB division region changed significantly, and the compactness of TAD structure decreased; The results of ATAC-seq showed that the open area and degree of chromatin in the deletion group decreased; 7778 differentially expressed mRNAs were found by RNA-seq. Our experimental results for the first time expounded the significance of Zbtb1 gene for T cell development, lymphocyte production and apoptosis from the aspects of chromosome spatial structure and chromatin opening degree, and provided relevant theoretical basis and data support for the in-depth study of related Zbtb1 genes in the future.
锌指和BTB结构域包含蛋白1（Zbtb1）是哺乳动物Zbtb基因家族的成员。通过Hi-C、ATAC-seq和RNA-seq技术对EL4细胞和Zbtb1缺陷型EL4细胞进行了一系列生物信息学分析。最后，Hi-C结果显示，缺失组中染色质相互作用强度随距离降低，染色体相互作用程度显著降低，AB分区区域发生显著变化，TAD结构的紧凑性降低；ATAC-seq结果显示，缺失组中染色质的开放区域和程度降低；通过RNA-seq发现了7778个差异表达的mRNA。我们的实验结果首次从染色体空间结构和染色质开放程度方面阐述了Zbtb1基因对T细胞发育、淋巴细胞产生和凋亡的意义，并为未来深入研究相关Zbtb1基因提供了相关理论依据和数据支持。
