Zhang Bing, Chen Si, Liu Jianxiu, Yan Yong-Bin, Chen Jingbo, Li Dandan, Liu Jin-Yuan
School of Life Sciences, Tsinghua University, Beijing, China.
College of Animal Science and Technology, Yangzhou University, Yangzhou, China.
Front Plant Sci. 2022 Apr 25;13:890980. doi: 10.3389/fpls.2022.890980. eCollection 2022.
Common bermudagrass ( L.) is an important perennial warm-season turfgrass species with great economic value. However, the reference genome is still deficient in , which severely impedes basic studies and breeding studies. In this study, a high-quality haplotype-resolved genome of cultivar Yangjiang was successfully assembled using a combination of multiple sequencing strategies. The assembled genome is approximately 1.01 Gb in size and is comprised of 36 pseudo chromosomes belonging to four haplotypes. In total, 76,879 protein-coding genes and 529,092 repeat sequences were annotated in the assembled genome. Evolution analysis indicated that underwent two rounds of whole-genome duplication events, whereas syntenic and transcriptome analysis revealed that global subgenome dominance was absent among the four haplotypes. Genome-wide gene family analyses further indicated that homologous recombination-regulating genes and tiller-angle-regulating genes all showed an adaptive evolution in , providing insights into genome-scale regulation of polyploid genome stability and prostrate growth. These results not only facilitate a better understanding of the complex genome composition and unique plant architectural characteristics of common bermudagrass, but also offer a valuable resource for comparative genome analyses of turfgrasses and other plant species.
狗牙根（Cynodon dactylon (L.) Pers.）是一种具有重要经济价值的多年生暖季型草坪草种。然而，其参考基因组仍然存在缺陷，这严重阻碍了基础研究和育种研究。在本研究中，利用多种测序策略成功组装了狗牙根品种阳江的高质量单倍型解析基因组。组装后的基因组大小约为1.01 Gb，由属于四个单倍型的36条假染色体组成。在组装的基因组中总共注释了76,879个蛋白质编码基因和529,092个重复序列。进化分析表明狗牙根经历了两轮全基因组复制事件，而共线性和转录组分析表明四个单倍型之间不存在全局亚基因组优势。全基因组基因家族分析进一步表明，同源重组调控基因和分蘖角调控基因在狗牙根中均表现出适应性进化，为多倍体基因组稳定性和匍匐生长的基因组规模调控提供了见解。这些结果不仅有助于更好地理解狗牙根复杂的基因组组成和独特的植物形态特征，也为草坪草和其他植物物种的比较基因组分析提供了宝贵的资源。
