一种用于数字分析的可控液滴合并系统的高效策略。

An efficient strategy for a controllable droplet merging system for digital analysis.

作者信息

Qiao Yi, Fu Jiye, Yang Fang, Duan Mengqin, Huang Mengting, Tu Jing, Lu Zuhong

机构信息

State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University Nanjing 210096 China

出版信息

RSC Adv. 2018 Oct 5;8(60):34343-34349. doi: 10.1039/c8ra06022c. eCollection 2018 Oct 4.

DOI:10.1039/c8ra06022c

PMID:35548645

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9086890/

Abstract

Droplet merging is an important part of droplet manipulation approaches. Droplet merging methods with expansions inside channels can merge droplets in pairs through simple structures. However, they have a low success rate of merging under unstable fluidic conditions since the one-to-one pairing strategy is sensitive to fluctuation. This study presents a one-to-a-cluster pairing strategy to improve the success rate of merging under fluctuation. The one-to-a-cluster method was suitable for digital analysis and droplet MDA was performed in merged droplets successfully.

摘要

液滴合并是液滴操控方法的重要组成部分。通道内带有扩张结构的液滴合并方法可通过简单结构成对合并液滴。然而，由于一对一配对策略对波动敏感，在不稳定的流体条件下它们的合并成功率较低。本研究提出了一种一对多簇配对策略，以提高波动条件下的合并成功率。一对多簇方法适用于数字分析，并且在合并后的液滴中成功进行了液滴多重置换扩增（MDA）。