Mo Juan-Fen, Wu Jia-Yuan, Zheng Li, Yu Ya-Wei, Zhang Tian-Xin, Guo Li, Bao Yi
The Key Laboratory, The Second Affiliated Hospital of Jiaxing University 1518 Huancheng North Road Jiaxing Zhejiang 314000 China
Department of Pathology, The Second Affiliated Hospital of Jiaxing University Jiaxing Zhejiang 314000 China.
RSC Adv. 2018 Sep 5;8(54):31194-31200. doi: 10.1039/c8ra05915b. eCollection 2018 Aug 30.
Polydatin (PD), a natural precursor of resveratrol, has been used to treat several diseases, such as cardiovascular diseases, hepatic diseases and various cancers. In this study, we aimed to investigate the protective effects and underlying mechanisms of PD on non-alcoholic fatty liver disease (NAFLD) using a high fat induced obese mice model. The studied subjects were randomly divided into a lean group, a high fat diet (HFD) group, and a high fat diet with PD (HFD + PD) group. The results showed that PD reduced the body weights in HFD mice. PD also downregulated the serum levels of triglyceride (TG), low density lipoprotein (LDL), aspartate aminotransferase (AST) and alanine aminotransferase (ALT), and upregulated high density lipoprotein (HDL). Moreover, PD significantly alleviated hepatocyte steatosis and reduced Gr-1 cells in the liver tissues of HFD mice. The mRNA levels of pro-inflammatory factors, such as monocyte chemoattractant protein-1 (MCP-1), tumor necrosis factor alpha (TNF-α), interleukin-6 (IL-6), S100A8 and S100A9 were significantly decreased in the liver tissues of HFD mice with PD treatment, and the downregulation of MCP-1 and S100A9 protein expressions was also observed. In conclusion, PD had beneficial roles in suppressing lipid accumulation in hepatocytes and anti-inflammatory responses in the liver tissue of obese associated NAFLD.
白藜芦醇苷（PD）是白藜芦醇的天然前体，已被用于治疗多种疾病，如心血管疾病、肝脏疾病和各种癌症。在本研究中，我们旨在使用高脂诱导的肥胖小鼠模型研究PD对非酒精性脂肪性肝病（NAFLD）的保护作用及其潜在机制。研究对象被随机分为瘦素组、高脂饮食（HFD）组和高脂饮食加PD（HFD+PD）组。结果表明，PD降低了HFD小鼠的体重。PD还下调了血清甘油三酯（TG）、低密度脂蛋白（LDL）、天冬氨酸转氨酶（AST）和丙氨酸转氨酶（ALT）的水平，并上调了高密度脂蛋白（HDL）。此外，PD显著减轻了HFD小鼠肝组织中的肝细胞脂肪变性，并减少了Gr-1细胞。在接受PD治疗的HFD小鼠肝组织中，促炎因子如单核细胞趋化蛋白-1（MCP-1）、肿瘤坏死因子α（TNF-α）、白细胞介素-6（IL-6）、S100A8和S100A9的mRNA水平显著降低，并且还观察到MCP-1和S100A9蛋白表达的下调。总之，PD在抑制肥胖相关NAFLD的肝细胞脂质积累和肝组织抗炎反应中具有有益作用。
