Université Clermont Auvergne, CNRS, Clermont Auvergne INP, Institut de Chimie de Clermont-Ferrand, F-63000 Clermont-Ferrand, France.
Université Clermont Auvergne, CHU Clermont-Ferrand, Clermont Auvergne INP, CNRS, Institut de Chimie de Clermont-Ferrand, F-63000 Clermont-Ferrand, France.
ACS Appl Bio Mater. 2022 Jun 20;5(6):2567-2575. doi: 10.1021/acsabm.2c00029. Epub 2022 May 13.
We investigate the adsorption of insulin onto PE and PVC materials by using HPLC measurements and computer simulations. We interpret the experiments by calculating the Gibbs free energy profiles during the adsorption process. The values of free energy of adsorption show a good agreement with the experimental measurements. The adsorption of insulin onto the different materials is characterized through the conformational changes with respect to its conformation in water and the interfacial regions, which are described by specific arrangements of polymer chains, water, insulin, and plasticizer molecules.
我们通过 HPLC 测量和计算机模拟研究了胰岛素在 PE 和 PVC 材料上的吸附。我们通过计算吸附过程中的吉布斯自由能曲线来解释实验。吸附自由能的值与实验测量值吻合较好。通过与胰岛素在水中的构象以及界面区域的构象相比，我们描述了不同材料对胰岛素的吸附特性，这是通过聚合物链、水、胰岛素和增塑剂分子的特定排列来实现的。
