Advanced Imaging Center, Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147.
Mol Biol Cell. 2022 May 15;33(6):tp1. doi: 10.1091/mbc.E21-10-0506.
While fluorescence microscopy has proven to be an exceedingly useful tool in bioscience, it is difficult to offer simultaneous high resolution, fast speed, large volume, and good biocompatibility in a single imaging technique. Thus, when determining the image data required to quantitatively test a complex biological hypothesis, it often becomes evident that multiple imaging techniques are necessary. Recent years have seen an explosion in development of novel fluorescence microscopy techniques, each of which features a unique suite of capabilities. In this Technical Perspective, we highlight recent studies to illustrate the benefits, and often the necessity, of combining multiple fluorescence microscopy modalities. We provide guidance in choosing optimal technique combinations to effectively address a biological question. Ultimately, we aim to promote a more well-rounded approach in designing fluorescence microscopy experiments, leading to more robust quantitative insight.
虽然荧光显微镜已被证明是生物科学中非常有用的工具，但很难在单一成像技术中同时提供高分辨率、快速速度、大容量和好的生物相容性。因此，在确定定量测试复杂生物假设所需的图像数据时，通常会发现需要多种成像技术。近年来，新型荧光显微镜技术的发展如雨后春笋般涌现，每种技术都具有独特的功能套件。在本技术观点中，我们强调了最近的研究，以说明结合多种荧光显微镜模式的好处，而且通常是必要性。我们提供了选择最佳技术组合的指导，以有效地解决生物学问题。最终，我们旨在促进设计荧光显微镜实验的更全面方法，从而获得更强大的定量洞察力。
