Laboratory of High-Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, 20892, USA.
Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, 20147, USA.
Histochem Cell Biol. 2022 Oct;158(4):301-323. doi: 10.1007/s00418-022-02147-4. Epub 2022 Aug 29.
Fluorescence microscopy is a highly effective tool for interrogating biological structure and function, particularly when imaging across multiple spatiotemporal scales. Here we survey recent innovations and applications in the relatively understudied area of multiscale fluorescence imaging of living samples. We discuss fundamental challenges in live multiscale imaging and describe successful examples that highlight the power of this approach. We attempt to synthesize general strategies from these test cases, aiming to help accelerate progress in this exciting area.
荧光显微镜是研究生物结构和功能的一种非常有效的工具，特别是在跨多个时空尺度成像时。在这里，我们调查了相对研究较少的活样本多尺度荧光成像领域的最新创新和应用。我们讨论了活多尺度成像中的基本挑战，并描述了成功的例子，突出了这种方法的优势。我们试图从这些案例中综合出一般策略，旨在帮助加速这一令人兴奋的领域的进展。
