Institute of Plant Protection, Guangxi Academy of Agricultural Sciences, Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China Ministry of Agriculture and Rural Affairs and Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pests, Nanning, Guangxi, 530007, China.
College of Life Sciences, Yangtze University, Jingzhou, Hubei, 434025, China.
Plant Dis. 2022 Dec;106(12):3154-3165. doi: 10.1094/PDIS-04-22-0765-RE. Epub 2022 Nov 20.
Pearl plum ( Lindl.) is mainly cultivated in Tian'e County in Guangxi Province, southern China. Anthracnose is a devastating disease on pearl plum, causing extensive leaf blight. Diseased leaves were sampled from 21 orchards in Tian'e County. Isolates were first screened for ones resembling , and 21 representative isolates were selected for sequencing of portions of the ribosomal internal transcribed spacer (ITS), the intergenic region of apn2 and MAT1-2-1 genes (ApMAT), actin (ACT), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), calmodulin (CAL), chitin synthase (CHS-1), and β-tubulin 2 (TUB2). Based on colony, conidial, and appressorial morphology and sequence analyses, the isolates associated with pearl plum anthracnose were identified as four species: (16 isolates), (3 isolates), (1 isolate), and (1 isolate). The results of pathogenicity tests showed that isolates of all four species were pathogenic to wounded leaves of pearl plum seedlings. In this study, we microscopically observed the infection processes of isolates of these four species on attached pearl plum leaves. For and , the entire infection processes took 120 h; for . and , it only took 72 h. This is the first report of and causing anthracnose on pearl plum worldwide, and also the first report of causing anthracnose on pearl plum in China.
珍珠李（Lindl.）主要在中国广西省田东县种植，是中国南方的一个重要经济作物。炭疽病是珍珠李的一种毁灭性病害，可引起广泛的叶片枯萎。从田东县的 21 个果园中采集了患病叶片。首先对这些叶片进行了类似于 的筛选，然后选择了 21 个具有代表性的分离物进行核糖体内部转录间隔区（ITS）、apn2 和 MAT1-2-1 基因（ApMAT）、肌动蛋白（ACT）、甘油醛-3-磷酸脱氢酶（GAPDH）、钙调蛋白（CAL）、几丁质合成酶（CHS-1）和β-微管蛋白 2（TUB2）基因片段的测序。根据菌落、分生孢子和附着孢形态和序列分析，与珍珠李炭疽病相关的 分离物被鉴定为四个种：（16 个分离物）、（3 个分离物）、（1 个分离物）和 （1 个分离物）。致病性测试的结果表明，这四个种的分离物均对珍珠李幼苗受伤叶片具有致病性。在这项研究中，我们用显微镜观察了这四个种的分离物在附着的珍珠李叶片上的感染过程。对于 和 ，整个感染过程需要 120 小时；对于. 和 ，只需要 72 小时。这是首次报道 和 在世界范围内引起珍珠李炭疽病，也是首次报道 在我国引起珍珠李炭疽病。
