Institute of Biotechnology, Federal University of Uberlândia, Acre Street, 2E building, Uberlândia, MG, 38405-319, Brazil.
Department of Genetics, Ecology and Evolution, Institute of Biological Sciences, Federal University of Minas Gerais, Presidente Antônio Carlos Avenue, 6627, Pampulha, Belo Horizonte, MG, 31270-901, Brazil.
BMC Microbiol. 2022 May 12;22(1):127. doi: 10.1186/s12866-022-02548-4.
The discovery of new molecules with antimicrobial properties has been a promising approach, mainly when related to substances produced by bacteria. The use of substances produced by bees has evidenced the antimicrobial action in different types of organisms. Thus, the use of bacteria isolated from larval food of stingless bees opens the way for the identification of the new molecules. The effect of supernatants produced by these bacteria was evaluated for their ability to inhibit the growth of bacteria of clinical interest. Furthermore, their effects were evaluated when used in synergy with antibiotics available in the pharmaceutical industry.
A few supernatants showed an inhibitory effect against susceptible and multiresistant strains in the PIC assay and the modulation assay. Emphasizing the inhibitory effect on multidrug-resistant strains, 7 showed an effect on multidrug-resistant Escherichia coli (APEC), Klebsiella pneumoniae carbapenemase (KPC), multidrug-resistant Pseudomonas aeruginosa, and multidrug-resistant Staphylococcus aureus (MRSA) in the PIC assay. Of the supernatants analyzed, some presented synergism for more than one species of multidrug-resistant bacteria. Nine had a synergistic effect with ampicillin on E. coli (APEC) or S. aureus (MRSA), 5 with penicillin G on E. coli (APEC) or KPC, and 3 with vancomycin on KPC.
In summary, the results indicate that supernatants produced from microorganisms can synthesize different classes of molecules with potent antibiotic activity against multiresistant bacteria. Thus, suggesting the use of these microorganisms for use clinical tests to isolate the molecules produced and their potential for use.
发现具有抗菌特性的新分子一直是一种很有前途的方法，尤其是与细菌产生的物质有关时。蜜蜂产生的物质在不同类型的生物体中都表现出了抗菌作用。因此，利用从无刺蜂幼虫食物中分离出的细菌来识别新分子成为可能。评估了这些细菌产生的上清液抑制有临床意义的细菌生长的能力。此外，还评估了它们与制药行业现有抗生素协同使用时的效果。
一些上清液在 PIC 测定和调制测定中对敏感和多药耐药菌株表现出抑制作用。强调对多药耐药大肠杆菌（APEC）、碳青霉烯酶肺炎克雷伯菌（KPC）、多药耐药铜绿假单胞菌和多药耐药金黄色葡萄球菌（MRSA）的抑制作用，7 种上清液在 PIC 测定中对多药耐药大肠杆菌（APEC）、多药耐药肺炎克雷伯菌（KPC）和多药耐药金黄色葡萄球菌（MRSA）有抑制作用。在分析的上清液中，有一些对不止一种多药耐药细菌具有协同作用。9 种上清液与氨苄西林对大肠杆菌（APEC）或金黄色葡萄球菌（MRSA）有协同作用，5 种与青霉素 G 对大肠杆菌（APEC）或 KPC 有协同作用，3 种与万古霉素对 KPC 有协同作用。
综上所述，结果表明，微生物产生的上清液可以合成不同类别的具有抗多药耐药菌活性的抗生素分子。因此，建议将这些微生物用于临床测试，以分离出产生的分子及其潜在用途。
