标准提取物和 fractions of L. 对两性霉素 B 敏感和临床两性霉素 B 耐药念珠菌的抑制作用

Inhibitory Effect of Standardized Extract and Fractions of L. on Nystatin Susceptible and Clinically Nystatin Resistant Candida .

机构信息

Department of Pharmacology, Medical Mycology and Bacteriology Research Center, Kerman University of Medical Sciences, Kerman, Iran.

Faculty of Pharmacy, Herbal and Traditional Medicines Research Center, Kerman University of Medical Sciences, Kerman, Iran.

出版信息

Curr Drug Discov Technol. 2022;19(5):e120522204695. doi: 10.2174/1570163819666220512164337.

DOI:10.2174/1570163819666220512164337

PMID:35549875

Abstract

INTRODUCTION

Candidiasis infection is caused by different species of Candida, which are characterized by host immunologic weakness. Black cumin seeds (Nigella sativa) have shown an inhibitory effect against Candida albicans. In this work, the inhibitory effect of standardized extract and different fractions of Nigella sativa seeds has been evaluated on both nystatin-susceptible and resistant strain of C. albicans.

MATERIALS AND METHODS

Canadida albicans (NSCA) with ATCC 76645 and nystatin-resistant Candida albicans (NRCA) were prepared from oral samples of HIV individuals. Total extract and different fractions of N. sativa were prepared using maceration and sonication methods. Thymoquinone (TQ) content of the plant was determined by spectrophotometric method. Total extract (TTE) and the fractions along with TQ were evaluated on NSCA and NRCA by the microdilution method.

RESULTS

TQ content of the plant was 0.92 ± 0.37g/100g dried extract. The least MIC and MFC (62.5 and 125 μg/ml, respectively) were due to petroleum ether fraction (PEF) against both NSCA and NRCA, followed by chloroform fraction (CHF) with MIC and MFC of 125 and 250 μg/ml, respectively. TQ exhibited MIC of 0.78 and 3.12 μg/ml against NSCA and NRCA, stronger than nystatin (MIC of 2 and 16 μg/ml, respectively). Thymoquinone was detected in the PEF and CHF.

CONCLUSION

Considering more inhibitory effects of PEF and CHF than TTE, we can conclude that active components of the plant belong to non-polar compounds. PEF showed identical inhibitory effects on NRCA and NSCA, which is a valuable result for finding novel medicaments against NRCA infections.

摘要

简介

念珠菌感染是由不同种的念珠菌引起的，这些念珠菌的特征是宿主免疫功能减弱。黑种草子（Nigella sativa）对白色念珠菌（Candida albicans）表现出抑制作用。在这项工作中，评估了黑种草子标准化提取物和不同馏分对耐制霉菌素的白色念珠菌（NRCA）和敏感的白色念珠菌（NSCA）的抑制作用。

材料和方法

从 HIV 个体的口腔样本中制备白色念珠菌（NSCA）和耐制霉菌素的白色念珠菌（NRCA）。使用浸渍法和超声法制备黑种草子的总提取物和不同馏分。用分光光度法测定植物中的百里醌（TQ）含量。用微量稀释法评价植物的总提取物（TTE）及其馏分以及 TQ 对 NSCA 和 NRCA 的作用。

结果

植物中 TQ 的含量为 0.92 ± 0.37g/100g 干提取物。最低的 MIC 和 MFC（分别为 62.5 和 125μg/ml）是石油醚馏分（PEF）对 NSCA 和 NRCA 的作用，其次是氯仿馏分（CHF），MIC 和 MFC 分别为 125 和 250μg/ml。TQ 对 NSCA 和 NRCA 的 MIC 分别为 0.78 和 3.12μg/ml，比制霉菌素（分别为 2 和 16μg/ml）更强。在 PEF 和 CHF 中检测到了百里醌。