Torabi Iman, Sharififar Fariba, Izadi Alireza, Ayatollahi Mousavi Seyed Amin
Department of Medical Parasitology & Mycology, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
Herbal and Traditional Medicines Research Center, Department of Pharmacognosy, School of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran.
Heliyon. 2022 Mar 9;8(3):e09073. doi: 10.1016/j.heliyon.2022.e09073. eCollection 2022 Mar.
and Purpose: Myrtle (.) is a medicinal herb that plays an essential role in treating fungal infections. The present study investigated the antifungal properties of different fractions of the leaf extract against (susceptible and resistant to nystatin).
Total extract (TE) and petroleum ether (PE), chloroform (CH), ethyl acetate (EA), and methanol (ME) fractions were prepared using the sonication method. The study used the standard strain sample (ATCC 76645) and nystatin-resistant from oral samples of HIV-infected individuals. The identification of resistant isolate was performed using phenotypic and molecular methods. Minimum inhibitory concentration (MIC) and Minimum fungicidal concentration (MFC) of the fractions along total extract were determined by microdilution method on nystatin-resistant and susceptible . The Folin-Ciocalteu method was used to determine the total phenolic content of the extract.
The extraction yield (w/w) was 13.50 for TE, 2.10 for PE, 2.23 for Ch, 2.14 for EA, and 10.03 for ME fractions. Chloroform extract showed good anti-candida activity against nystatin susceptible and resistant (62.5 μg/mL). Ethyl acetate fraction exhibited the greatest MIC against nystatin susceptible and resistant (250 μg/mL). The MIC value of fluconazole was >64 μg/mL for both susceptible and -resistant strains. The amount of phenolic compounds of the total extract was reported to be equal to 5.4%, equivalent to gallic acid.
Results revealed that the PE and CH fractions showed greater antifungal effects than the total extract against both susceptible and resistant strains of It can conclude that active antifungal compounds of the plant belong to a specific group of metabolites, which according to the type of solvent, probably have non-polar nature. Further separation is carrying out.
桃金娘是一种草药，在治疗真菌感染中发挥着重要作用。本研究调查了叶提取物不同馏分对白色念珠菌（对制霉菌素敏感和耐药）的抗真菌特性。
采用超声法制备总提取物（TE）以及石油醚（PE）、氯仿（CH）、乙酸乙酯（EA）和甲醇（ME）馏分。本研究使用标准菌株样本（ATCC 76645）以及来自HIV感染个体口腔样本的耐制霉菌素白色念珠菌。采用表型和分子方法对耐药分离株进行鉴定。通过微量稀释法测定各馏分以及总提取物对耐制霉菌素和敏感白色念珠菌的最低抑菌浓度（MIC）和最低杀菌浓度（MFC）。采用福林 - 西奥尔特法测定提取物的总酚含量。
TE的提取率（w/w）为13.50，PE为2.10，Ch为2.23，EA为2.14，ME馏分为10.03。氯仿提取物对制霉菌素敏感和耐药白色念珠菌均显示出良好的抗念珠菌活性（62.5 μg/mL）。乙酸乙酯馏分对制霉菌素敏感和耐药白色念珠菌的MIC最高（250 μg/mL）。氟康唑对敏感和耐药菌株的MIC值均>64 μg/mL。据报道，总提取物中酚类化合物的含量相当于5.4%，等同于没食子酸。
结果表明，PE和CH馏分对白色念珠菌敏感和耐药菌株均显示出比总提取物更强的抗真菌作用。可以得出结论，该植物的活性抗真菌化合物属于特定的代谢物组，根据溶剂类型，可能具有非极性性质。进一步的分离正在进行。
