Pankongadisak Porntipa, Suwantong Orawan
School of Science, Mae Fah Luang University Tasud, Muang Chiang Rai 57100 Thailand
Center for Chemical Innovation for Sustainability (CIS), Mae Fah Luang University Tasud, Muang Chiang Rai 57100 Thailand.
RSC Adv. 2018 Dec 3;8(70):40219-40231. doi: 10.1039/c8ra07255h. eCollection 2018 Nov 28.
In this study, hydrogels that were thermosensitive at body temperature were developed using chitosan (CS)/silk sericin (SS)/β-glycerophosphate (β-GP) loaded with longan seed extract (LE) for use in bone tissue engineering. These hydrogels were transformed into a gel at 37 °C within 10 min interactions between CS and β-GP. The incorporation of SS resulted in a shorter gelation time of 5-7 min. The morphological structure of the thermosensitive CS/β-GP hydrogels exhibited an irregular pore structure, whereas the morphological structure of the thermosensitive CS/SS/β-GP hydrogels became more slender and porous. The incorporation of SS affected the network structure of the CS hydrogels, which degraded more rapidly. Moreover, the cumulative amounts of both gallic acid (GA) and ellagic acid (EA) released from the hydrogels loaded with LE increased with an increase in the SS content. Finally, these thermosensitive hydrogels were non-toxic to both a mouse fibroblast cell line (NCTC clone 929) and a mouse osteoblast cell line (MC3T3-E1) and promoted the attachment of MC3T3-E1 cells to the surface of the hydrogels. Therefore, these thermosensitive hydrogels might be a promising candidate for bone tissue engineering.
在本研究中，使用负载龙眼籽提取物（LE）的壳聚糖（CS）/丝胶蛋白（SS）/β-甘油磷酸酯（β-GP）制备了在体温下具有热敏性的水凝胶，用于骨组织工程。由于CS与β-GP之间的相互作用，这些水凝胶在37℃下10分钟内转变为凝胶。加入SS后凝胶化时间缩短至5 - 7分钟。热敏性CS/β-GP水凝胶的形态结构呈现不规则孔隙结构，而热敏性CS/SS/β-GP水凝胶的形态结构变得更细长且多孔。SS的加入影响了CS水凝胶的网络结构，使其降解更快。此外，负载LE的水凝胶中没食子酸（GA）和鞣花酸（EA）的累积释放量随SS含量的增加而增加。最后，这些热敏性水凝胶对小鼠成纤维细胞系（NCTC克隆929）和小鼠成骨细胞系（MC3T3-E1）均无毒，并促进MC3T3-E1细胞附着于水凝胶表面。因此，这些热敏性水凝胶可能是骨组织工程中有前景的候选材料。
