Kalita Himadri, Hazarika Ankita, Kandimalla Raghuram, Kalita Sanjeeb, Devi Rajlakshmi
Life Sciences Division, Institute of Advanced Study in Science and Technology Guwahati Assam India
Drug Discovery Laboratory, Institute of Advanced Study in Science and Technology Guwahati Assam 781035 India
RSC Adv. 2018 Oct 31;8(64):36791-36801. doi: 10.1039/c8ra04470h. eCollection 2018 Oct 26.
The search to develop an ideal suture material encourages us to explore novel suture biomaterials with superior characteristics to the current commercially available products. Surgical sutures play a crucial role in the development of post-operative wound infection by acting as a substrate for biofilm formation which leads to dehisced wounds. In this context, the present invention meets this need by fabricating banana () fibre into an advanced antimicrobials releasing suture biomaterial (BSc) for the prevention of post-operative wound infection. Suture material developed from banana pseudo stem fiber was impregnated with chloramphenicol, clotrimazole and growth factors with the aid of a hydro-gel system. The fabricated suture material was found to be biocompatible towards human erythrocytes and L929 mouse fibroblast cells. BSc exhibited promising physico-chemical characteristics which were comparable to the commercially available silk fibroin (BMSF) suture. BSc displayed a biphasic release pattern with sustained release of chloramphenicol for up to 140 h. Apart from being environment friendly and having a facile fabrication method, this advanced suture biomaterial showed broad spectrum antimicrobial activity against bacterial and fungal pathogens. BSc successfully impeded biofilm formation on its surface, as is evident from the confocal microscopy analysis. This contributes to superior wound healing efficacy in terms of reduced microbial burden and a subsequent decrease in the inflammatory cytokine levels. Histopathological observations further supported the pronounced healing efficacy of BSc sutured wounds. The findings of this study establish the banana pseudo stem fiber as a novel advanced suture biomaterial to prevent post-operative wound infections.
开发理想缝合材料的探索促使我们去探寻具有比现有商业产品更优异特性的新型缝合生物材料。手术缝线在术后伤口感染的发展中起着关键作用，因为它充当了生物膜形成的基质，进而导致伤口裂开。在此背景下，本发明通过将香蕉（）纤维制成一种先进的抗菌释放缝合生物材料（BSc）来满足这一需求，以预防术后伤口感染。由香蕉假茎纤维开发的缝合材料借助水凝胶系统浸渍了氯霉素、克霉唑和生长因子。发现制成的缝合材料对人红细胞和L929小鼠成纤维细胞具有生物相容性。BSc展现出了有前景的物理化学特性，可与市售丝素蛋白（BMSF）缝合线相媲美。BSc呈现出双相释放模式，氯霉素可持续释放长达140小时。除了环境友好且制备方法简便外，这种先进的缝合生物材料对细菌和真菌病原体具有广谱抗菌活性。从共聚焦显微镜分析可以明显看出，BSc成功地阻止了其表面生物膜的形成。这在减少微生物负荷以及随后降低炎性细胞因子水平方面有助于提高伤口愈合效果。组织病理学观察进一步支持了BSc缝合伤口的显著愈合效果。本研究结果确立了香蕉假茎纤维作为一种新型先进缝合生物材料用于预防术后伤口感染。
