Liu Jie, Wang Huailing, Liu Xiaoyu, Zhang Guohao, Liu Zhigang
Department of Allergy, The Third Affiliated Hospital of Shenzhen University Shenzhen 518020 China
The Research Center of Allergy & Immunology, Shenzhen University School of Medicine Shenzhen 518060 China.
RSC Adv. 2018 Nov 15;8(67):38529-38537. doi: 10.1039/c8ra06575f. eCollection 2018 Nov 14.
Recent reports have indicated that the ingredients in Chinese liquor possess multiple bioactivities. The objective of this study was to evaluate the potential effects of Chinese liquor extract (CME) on the resistance to inflammation in mononuclear macrophages (RAW 264.7 cell line) and aging in (). The results showed that CME suppressed key lipopolysaccharide (LPS)-induced pro-inflammatory mediators, tumor necrosis factor-α, and nitric oxide . Furthermore, CME inhibited activation of mitogen-activated protein kinase (MAPK) and phosphatidylinositol-3 kinase (PI3K)/AKT pathways in LPS-stimulated cells. Further studies also showed that CME improved stress resistance of nematodes under infection conditions. Moreover, CME increased the expression of immune-related genes, such as . Based on these results, our findings provide mechanistic insights about the protection provided by CME against LPS-induced inflammation in RAW 264.7 cells, namely, inhibition of MAPK and PI3K/AKT pathways, as well as its capability against - and -induced aging in .
最近的报告表明，白酒中的成分具有多种生物活性。本研究的目的是评估白酒提取物（CME）对单核巨噬细胞（RAW 264.7细胞系）炎症抗性和（此处原文缺失相关内容）衰老的潜在影响。结果表明，CME抑制了关键的脂多糖（LPS）诱导的促炎介质、肿瘤坏死因子-α和一氧化氮。此外，CME抑制了LPS刺激细胞中丝裂原活化蛋白激酶（MAPK）和磷脂酰肌醇-3激酶（PI3K）/AKT途径的激活。进一步的研究还表明，CME提高了感染条件下线虫的抗应激能力。此外，CME增加了免疫相关基因的表达，如。基于这些结果，我们的研究结果提供了关于CME对RAW 264.7细胞中LPS诱导的炎症的保护机制的见解，即抑制MAPK和PI3K/AKT途径，以及其对抗（此处原文缺失相关内容）和（此处原文缺失相关内容）诱导的衰老的能力。
