Division of Reproductive Endocrinology, MetroHealth Medical Center, Parma, Ohio.
Androlab Inc, Chicago, Illinois.
F S Sci. 2022 Feb;3(1):18-20. doi: 10.1016/j.xfss.2021.11.001. Epub 2021 Nov 9.
The first two editions of the World Health Organization laboratory manual described the determination of live spermatozoa by a dye exclusion method as a sperm "viability" test, whereas subsequent editions classified it as a "vitality" test, without providing an explanation for the reclassification. Additionally, the hypo-osmotic swelling (HOS) test, which assesses the functional integrity of the human sperm membrane, was placed in the same category as the dye exclusion test. Although the two terms might seem synonymous, the term "vitality" merely means "alive," whereas "viability" assesses qualities or physiological functions of a living entity. After comparing the morphological, physiological, and clinical findings obtained from dye exclusion testing vs. the HOS test, we conclude that the HOS test should be classified as a viability test, not merely as a vitality test.
世界卫生组织实验室手册的前两版将通过染料排除法来测定活精子的方法描述为一种精子“活力”检测，而随后的版本将其归类为“生存力”检测，并未对重新分类做出解释。此外，评估人类精子膜功能完整性的低渗肿胀（HOS）检测也被归入与染料排除检测相同的类别。虽然这两个术语似乎可以互换，但“活力”一词仅表示“活着”，而“生存力”则评估活生物体的特性或生理功能。通过比较染料排除检测与 HOS 检测获得的形态学、生理学和临床发现，我们得出结论，HOS 检测应归类为生存力检测，而不仅仅是活力检测。
