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一个抗性基因同源物目录和一个染色体尺度的参考序列支持冬小麦抗性基因定位。

A catalogue of resistance gene homologs and a chromosome-scale reference sequence support resistance gene mapping in winter wheat.

机构信息

Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, Seeland, Germany.

Limagrain GmbH, Peine-Rosenthal, Germany.

出版信息

Plant Biotechnol J. 2022 Sep;20(9):1730-1742. doi: 10.1111/pbi.13843. Epub 2022 May 30.

DOI:10.1111/pbi.13843
PMID:35562859
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9398310/
Abstract

A resistance gene atlas is an integral component of the breeder's arsenal in the fight against evolving pathogens. Thanks to high-throughput sequencing, catalogues of resistance genes can be assembled even in crop species with large and polyploid genomes. Here, we report on capture sequencing and assembly of resistance gene homologs in a diversity panel of 907 winter wheat genotypes comprising ex situ genebank accessions and current elite cultivars. In addition, we use accurate long-read sequencing and chromosome conformation capture sequencing to construct a chromosome-scale genome sequence assembly of cv. Attraktion, an elite variety representative of European winter wheat. We illustrate the value of our resource for breeders and geneticists by (i) comparing the resistance gene complements in plant genetic resources and elite varieties and (ii) conducting genome-wide associations scans (GWAS) for the fungal diseases yellow rust and leaf rust using reference-based and reference-free GWAS approaches. The gene content under GWAS peaks was scrutinized in the assembly of cv. Attraktion.

摘要

抗性基因图谱是培育者对抗不断进化的病原体的武器库中的一个重要组成部分。由于高通量测序的出现，即使在基因组庞大且为多倍体的作物物种中，也可以对抗性基因进行编目。在这里，我们报告了对包含来自于种质库的外存材料和当前优良品种的 907 个冬小麦基因型多样性面板的抗性基因同源物的捕获测序和组装。此外，我们使用准确的长读测序和染色体构象捕获测序，构建了欧洲冬小麦代表品种 Attraktion 的染色体级别的基因组序列组装。我们通过（i）比较植物遗传资源和优良品种中的抗性基因组成，以及（ii）使用基于参考和无参考的 GWAS 方法对黄锈病和叶锈病这两种真菌病进行全基因组关联分析 (GWAS)，来展示我们的资源对培育者和遗传学家的价值。在 Attraktion 的组装中，对 GWAS 峰下的基因含量进行了仔细研究。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a0d5/11384618/95cfea009378/PBI-20-1730-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a0d5/11384618/8e2b01836756/PBI-20-1730-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a0d5/11384618/06a876f648eb/PBI-20-1730-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a0d5/11384618/0dfa1f337727/PBI-20-1730-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a0d5/11384618/2d7b3a82fd66/PBI-20-1730-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a0d5/11384618/33ebd5c3af7f/PBI-20-1730-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a0d5/11384618/1640e79ce5b8/PBI-20-1730-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a0d5/11384618/56dabb5c125a/PBI-20-1730-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a0d5/11384618/95cfea009378/PBI-20-1730-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a0d5/11384618/8e2b01836756/PBI-20-1730-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a0d5/11384618/06a876f648eb/PBI-20-1730-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a0d5/11384618/0dfa1f337727/PBI-20-1730-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a0d5/11384618/2d7b3a82fd66/PBI-20-1730-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a0d5/11384618/33ebd5c3af7f/PBI-20-1730-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a0d5/11384618/1640e79ce5b8/PBI-20-1730-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a0d5/11384618/56dabb5c125a/PBI-20-1730-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a0d5/11384618/95cfea009378/PBI-20-1730-g006.jpg

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