The Transcriptome and Metabolome Reveal the Potential Mechanism of Lodging Resistance in Intergeneric Hybrids between and .

Lodging is one of the main reasons for the reduction in seed yield and is the limitation of mechanized harvesting in . The dissection of the regulatory mechanism of lodging resistance is an important goal in . In this study, the lodging resistant line, YG689, derived from the hybridization between cv. Zhongyou 821 (ZY821) and , was used to dissect the regulation mechanism of hard stem formation by integrating anatomical structure, transcriptome and metabolome analyses. It was shown that the lignocellulose content of YG689 is higher than that of ZY821, and some differentially expressed genes (DEGs) involved in the lignocellulose synthesis pathway were revealed by transcriptome analyses. Meanwhile, GC-TOF-MS and UPLC-QTOF-MS identified 40, 54, and 31 differential metabolites in the bolting stage, first flower stage, and the final flower stage. The differential accumulation of these metabolites might be associated with the lignocellulose biosynthesis in . Finally, some important genes that regulate the metabolic pathway of lignocellulose biosynthesis, such as , , and NewGene_216 were identified in through the combination of transcriptomics and metabolomics data. The present results explored the potential regulatory mechanism of lignocellulose biosynthesis, which provided a new clue for the breeding of with lodging resistance in the future.