Garvicins AG1 and AG2: Two Novel Class IId Bacteriocins of Lg-Granada.

causes infectious diseases in animals and is considered an emerging zoonotic pathogen involved in human clinical conditions. In silico analysis of plasmid pLG50 of Lg-Granada, an isolate from a patient with endocarditis, revealed the presence of two gene clusters (46-47 and 48-49), each one encoding a novel putative bacteriocin, i.e., garvicin AG1 (GarAG1; 46) and garvicin AG2 (GarAG2; 48), and their corresponding immunity proteins (47 and 49). The chemically synthesised bacteriocins GarAG1 and GarAG2 presented inhibitory activity against pathogenic strains, with AG2 also being active against , and . Genetic organisation, amino acid sequences and antimicrobial activities of GarAG1 and GarAG2 indicate that they belong to linear non-pediocin-like one-peptide class IId bacteriocins. Gram-positive bacteria that were sensitive to GarAG2 were also able to ferment mannose, suggesting that this bacteriocin could use the mannose phosphotransferase transport system (Man-PTS) involved in mannose uptake as a receptor in sensitive strains. Intriguingly, GarAG1 and GarAG2 were highly active against their own host, Lg-Granada, which could be envisaged as a new strategy to combat pathogens via their own weapons.