• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

营养不良老年大鼠骨骼肌蛋白质组学特征分析。

Characterization of the Skeletal Muscle Proteome in Undernourished Old Rats.

机构信息

Human Nutrition Unit, INRAE, Auvergne Human Nutrition Research Center, Clermont Auvergne University, 63000 Clermont-Ferrand, France.

Animal Products Quality Unit (QuaPA), INRAE, 63122 Clermont-Ferrand, France.

出版信息

Int J Mol Sci. 2022 Apr 26;23(9):4762. doi: 10.3390/ijms23094762.

DOI:10.3390/ijms23094762
PMID:35563153
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9101871/
Abstract

Aging is associated with a progressive loss of skeletal muscle mass and function termed sarcopenia. Various metabolic alterations that occur with aging also increase the risk of undernutrition, which can worsen age-related sarcopenia. However, the impact of undernutrition on aged skeletal muscle remains largely under-researched. To build a deeper understanding of the cellular and molecular mechanisms underlying age-related sarcopenia, we characterized the undernutrition-induced changes in the skeletal muscle proteome in old rats. For this study, 20-month-old male rats were fed 50% or 100% of their spontaneous intake for 12 weeks, and proteomic analysis was performed on both slow- and fast-twitch muscles. Proteomic profiling of undernourished aged skeletal muscle revealed that undernutrition has profound effects on muscle proteome independently of its effect on muscle mass. Undernutrition-induced changes in muscle proteome appear to be muscle-type-specific: slow-twitch muscle showed a broad pattern of differential expression in proteins important for energy metabolism, whereas fast-twitch muscle mainly showed changes in protein turnover between undernourished and control rats. This first proteomic analysis of undernourished aged skeletal muscle provides new molecular-level insight to explain phenotypic changes in undernourished aged muscle. We anticipate this work as a starting point to define new biomarkers associated with undernutrition-induced muscle loss in the elderly.

摘要

衰老是与骨骼肌质量和功能逐渐丧失相关的，这种现象被称为肌肉减少症。随着年龄的增长，各种代谢变化也会增加营养不良的风险，而营养不良会使与年龄相关的肌肉减少症恶化。然而，营养不良对老年骨骼肌的影响在很大程度上仍未得到充分研究。为了更深入地了解与年龄相关的肌肉减少症的细胞和分子机制，我们对老年大鼠骨骼肌中的营养不良诱导的蛋白质组变化进行了特征描述。在这项研究中，20 月龄的雄性大鼠接受 50%或 100%的自发摄入量喂养 12 周，对慢肌和快肌进行蛋白质组分析。对营养不良的老年骨骼肌进行蛋白质组学分析表明，营养不良对肌肉蛋白质组的影响独立于其对肌肉质量的影响。肌肉蛋白质组的营养不良诱导变化似乎具有肌肉类型特异性：慢肌中与能量代谢有关的蛋白质的差异表达广泛，而快肌中主要表现为营养不良和对照大鼠之间的蛋白质周转变化。这项对营养不良的老年骨骼肌的首次蛋白质组学分析为解释营养不良的老年肌肉的表型变化提供了新的分子水平的见解。我们期望这项工作可以作为一个起点，定义与老年人中由营养不良引起的肌肉丧失相关的新生物标志物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad03/9101871/21c669ff8ee9/ijms-23-04762-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad03/9101871/67bbc3fc01ea/ijms-23-04762-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad03/9101871/b3cc554ba6b1/ijms-23-04762-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad03/9101871/89939d85af54/ijms-23-04762-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad03/9101871/14a16c7cf7d5/ijms-23-04762-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad03/9101871/52b357764db8/ijms-23-04762-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad03/9101871/85da29960deb/ijms-23-04762-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad03/9101871/6ffb3d37ccad/ijms-23-04762-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad03/9101871/21c669ff8ee9/ijms-23-04762-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad03/9101871/67bbc3fc01ea/ijms-23-04762-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad03/9101871/b3cc554ba6b1/ijms-23-04762-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad03/9101871/89939d85af54/ijms-23-04762-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad03/9101871/14a16c7cf7d5/ijms-23-04762-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad03/9101871/52b357764db8/ijms-23-04762-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad03/9101871/85da29960deb/ijms-23-04762-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad03/9101871/6ffb3d37ccad/ijms-23-04762-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad03/9101871/21c669ff8ee9/ijms-23-04762-g008.jpg

相似文献

1
Characterization of the Skeletal Muscle Proteome in Undernourished Old Rats.营养不良老年大鼠骨骼肌蛋白质组学特征分析。
Int J Mol Sci. 2022 Apr 26;23(9):4762. doi: 10.3390/ijms23094762.
2
Novel Sarcopenia-related Alterations in Sarcomeric Protein Post-translational Modifications (PTMs) in Skeletal Muscles Identified by Top-down Proteomics.通过自上而下的蛋白质组学鉴定骨骼肌肌节蛋白翻译后修饰（PTMs）中的新型与肌肉减少症相关的改变。
Mol Cell Proteomics. 2018 Jan;17(1):134-145. doi: 10.1074/mcp.RA117.000124. Epub 2017 Oct 18.
3
Drastic increase of myosin light chain MLC-2 in senescent skeletal muscle indicates fast-to-slow fibre transition in sarcopenia of old age.衰老骨骼肌中肌球蛋白轻链MLC-2的急剧增加表明老年肌肉减少症中快肌纤维向慢肌纤维的转变。
Eur J Cell Biol. 2009 Nov;88(11):685-700. doi: 10.1016/j.ejcb.2009.06.004. Epub 2009 Jul 19.
4
Fiber-Type Shifting in Sarcopenia of Old Age: Proteomic Profiling of the Contractile Apparatus of Skeletal Muscles.衰老性肌肉减少症中的纤维类型转变：骨骼肌收缩装置的蛋白质组学分析。
Int J Mol Sci. 2023 Jan 26;24(3):2415. doi: 10.3390/ijms24032415.
5
Redox responses are preserved across muscle fibres with differential susceptibility to aging.氧化还原反应在具有不同衰老易感性的肌肉纤维中得以保留。
J Proteomics. 2018 Apr 15;177:112-123. doi: 10.1016/j.jprot.2018.02.015. Epub 2018 Feb 10.
6
Comparative proteomic analysis of the aging soleus and extensor digitorum longus rat muscles using TMT labeling and mass spectrometry.采用 TMT 标记和质谱技术对衰老比目鱼肌和趾长伸肌的比较蛋白质组学分析。
J Proteome Res. 2013 Oct 4;12(10):4532-46. doi: 10.1021/pr400644x. Epub 2013 Sep 25.
7
Proteomic profiling reveals a severely perturbed protein expression pattern in aged skeletal muscle.蛋白质组分析揭示了老年骨骼肌中严重紊乱的蛋白质表达模式。
Int J Mol Med. 2007 Aug;20(2):145-53.
8
Muscle Proteomic and Transcriptomic Profiling of Healthy Aging and Metabolic Syndrome in Men.男性健康衰老和代谢综合征的肌肉蛋白质组学和转录组学分析。
Int J Mol Sci. 2021 Apr 19;22(8):4205. doi: 10.3390/ijms22084205.
9
Proteomic changes in skeletal muscle of aged rats in response to resistance training.老年大鼠骨骼肌对抗阻训练的蛋白质组学变化。
Cell Biochem Funct. 2020 Jun;38(4):500-509. doi: 10.1002/cbf.3497. Epub 2020 Jan 27.
10
Genomic and proteomic profiling reveals reduced mitochondrial function and disruption of the neuromuscular junction driving rat sarcopenia.基因组和蛋白质组分析揭示了线粒体功能降低和神经肌肉接头破坏导致大鼠骨骼肌减少。
Mol Cell Biol. 2013 Jan;33(2):194-212. doi: 10.1128/MCB.01036-12. Epub 2012 Oct 29.

引用本文的文献

1
Combined Nutrition with Exercise: Fueling the Fight Against Sarcopenia Through a Bibliometric Analysis and Review.营养与运动相结合：通过文献计量分析与综述为对抗肌肉减少症提供动力
Int J Gen Med. 2024 May 3;17:1861-1876. doi: 10.2147/IJGM.S462594. eCollection 2024.
2
Highlights in Pathophysiology of the Musculoskeletal System.肌肉骨骼系统病理生理学要点。
Int J Mol Sci. 2023 Mar 29;24(7):6412. doi: 10.3390/ijms24076412.
3
Fiber-Type Shifting in Sarcopenia of Old Age: Proteomic Profiling of the Contractile Apparatus of Skeletal Muscles.

本文引用的文献

1
Quantitative Proteomics Analysis for the Identification of Differential Protein Expression in Calf Muscles between Young and Old SD Rats Using Mass Spectrometry.定量蛋白质组学分析：利用质谱法鉴定年轻与老年SD大鼠小腿肌肉中差异蛋白质表达
ACS Omega. 2021 Mar 10;6(11):7422-7433. doi: 10.1021/acsomega.0c05821. eCollection 2021 Mar 23.
2
Reduced metabolic capacity in fast and slow skeletal muscle via oxidative stress and the energy-sensing of AMPK/SIRT1 in malnutrition.营养不良时通过氧化应激和 AMPK/SIRT1 的能量感应降低快肌和慢肌的代谢能力。
Physiol Rep. 2021 Mar;9(5):e14763. doi: 10.14814/phy2.14763.
3
Mechanisms of muscle atrophy and hypertrophy: implications in health and disease.
衰老性肌肉减少症中的纤维类型转变：骨骼肌收缩装置的蛋白质组学分析。
Int J Mol Sci. 2023 Jan 26;24(3):2415. doi: 10.3390/ijms24032415.
肌肉萎缩和肥大的机制：对健康和疾病的影响。
Nat Commun. 2021 Jan 12;12(1):330. doi: 10.1038/s41467-020-20123-1.
4
Marked Increased Production of Acute Phase Reactants by Skeletal Muscle during Cancer Cachexia.癌症恶病质期间骨骼肌急性期反应物的产生显著增加。
Cancers (Basel). 2020 Oct 31;12(11):3221. doi: 10.3390/cancers12113221.
5
Signaling Pathways That Control Muscle Mass.控制肌肉质量的信号通路。
Int J Mol Sci. 2020 Jul 4;21(13):4759. doi: 10.3390/ijms21134759.
6
Discovery proteomics in aging human skeletal muscle finds change in spliceosome, immunity, proteostasis and mitochondria.衰老人类骨骼肌中的发现蛋白质组学发现剪接体、免疫、蛋白质稳态和线粒体的变化。
Elife. 2019 Oct 23;8:e49874. doi: 10.7554/eLife.49874.
7
HuR counteracts miR-330 to promote STAT3 translation during inflammation-induced muscle wasting.HuR 拮抗 miR-330 促进炎症诱导的肌肉减少症中的 STAT3 翻译。
Proc Natl Acad Sci U S A. 2019 Aug 27;116(35):17261-17270. doi: 10.1073/pnas.1905172116. Epub 2019 Aug 12.
8
Malnutrition and sarcopenia.营养不良和肌肉减少症。
Aging Clin Exp Res. 2019 Jun;31(6):793-798. doi: 10.1007/s40520-019-01170-1. Epub 2019 May 30.
9
The Challenge of Managing Undernutrition in Older People with Frailty.老年人虚弱与营养不良管理的挑战。
Nutrients. 2019 Apr 10;11(4):808. doi: 10.3390/nu11040808.
10
PDK4 drives metabolic alterations and muscle atrophy in cancer cachexia.PDK4 驱动癌症恶病质中的代谢改变和肌肉萎缩。
FASEB J. 2019 Jun;33(6):7778-7790. doi: 10.1096/fj.201802799R. Epub 2019 Mar 20.