Katz M E, Ferguson J, Reed S I
Genetics. 1987 Apr;115(4):627-36. doi: 10.1093/genetics/115.4.627.
A procedure was devised to isolate mutations that could restore conjugational competence to temperature sensitive ste mutants and simultaneously confer temperature-sensitive lethal growth phenotypes. Three such mutations, falling into two complementation groups, were identified on the basis of suppression of ste5 alleles. These same mutations were later shown to be capable of suppressing ste4 and ste7 alleles. Five mutations in a single complementation group were isolated as suppressors of ste2 alleles. None of the mutations described in this study conferred a homogeneous cell cycle arrest phenotype, and all were shown to define complementation groups distinct from those previously identified in studies of cell division cycle (cdc) mutations. In no instance did pseudoreversion appear to be achieved by mutational G1 arrest of ste mutant cells. Instead, it is proposed that the mutations restore conjugation by reestablishing the normal pheromone response.
设计了一种程序来分离那些能够恢复温度敏感型ste突变体的接合能力并同时赋予温度敏感型致死生长表型的突变。基于对ste5等位基因的抑制作用，鉴定出了三个属于两个互补群的此类突变。后来发现这些相同的突变能够抑制ste4和ste7等位基因。在一个单一互补群中分离出五个突变作为ste2等位基因的抑制子。本研究中描述的突变均未赋予均匀的细胞周期停滞表型，并且所有这些突变都被证明定义了与先前在细胞分裂周期（cdc）突变研究中鉴定出的互补群不同的互补群。在任何情况下，似乎都不是通过ste突变体细胞的G1期突变停滞来实现假回复的。相反，有人提出这些突变通过重新建立正常的信息素反应来恢复接合作用。
