对α交配型因子具有抗性的酿酒酵母突变体。
Mutants of Saccharomyces cerevisiae resistant to the alpha mating-type factor.
作者信息
Manney T R, Woods V
出版信息
Genetics. 1976 Apr;82(4):639-44. doi: 10.1093/genetics/82.4.639.
Abstract
Mutants that are resistant to alpha-factor have been isolated from a mating-type haploid strains of yeast by direct selection on agar medium containing partially purified alpha-factor. All resistant mutants isolated were found to be sterile. They were characterized and compared with mutants previously isolated as non-mating. Among 93 able to mate at low frequency and to sporulate, none showed linkage to the mating-type locus. The results support the hypothesis that the response to alpha-factor by cells of mating-type a is essential for mating.
摘要
通过在含有部分纯化α因子的琼脂培养基上直接筛选，从酵母的交配型单倍体菌株中分离出了对α因子具有抗性的突变体。所有分离出的抗性突变体均被发现是不育的。对它们进行了表征，并与先前作为不交配而分离出的突变体进行了比较。在93个能够低频率交配并形成孢子的突变体中，没有一个显示出与交配型基因座的连锁关系。这些结果支持了这样的假说，即交配型a细胞对α因子的反应对于交配至关重要。
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本文引用的文献
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