含磷羟基磷灰石纳米颗粒（nHAP）作为新型纳米肥料的绿色合成：对石榴（L.）的初步评估

Green Synthesis of Phosphorous-Containing Hydroxyapatite Nanoparticles (nHAP) as a Novel Nano-Fertilizer: Preliminary Assessment on Pomegranate ( L.).

作者信息

Abdelmigid Hala M, Morsi Maissa M, Hussien Nahed Ahmed, Alyamani Amal Ahmed, Alhuthal Nawal Abdallah, Albukhaty Salim

机构信息

Department of Biotechnology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia.

Department of Biology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia.

出版信息

Nanomaterials (Basel). 2022 May 1;12(9):1527. doi: 10.3390/nano12091527.

Nano-fertilizers are innovative materials created by nanotechnology methodologies that may potentially replace traditional fertilizers due to their rapid absorption and controlled distribution of nutrients in plants. In the current study, phosphorous-containing hydroxyapatite nanoparticles (nHAP) were synthesized as a novel phosphorus nano-fertilizer using an environmentally friendly green synthesis approach using pomegranate peel (PPE) and coffee ground (CE) extracts. nHAPs were physicochemically characterized and biologically evaluated utilizing the analysis of biochemical parameters such as photosynthetic activity, carbohydrate levels, metabolites, and biocompatibility changes in L. Cytocompatibility with mammalian cells was also investigated based on MTT assay on a Vero cell line. Dynamic light scattering (DLS) and zeta potential analysis were used to characterize the nHAPs for size and surface charge as well as morphology using scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The nHAPs were found to have different shapes with average sizes of 229.6 nm, 120.6 nm (nHAPs_PPE) and 167.5 nm, 153 nm (nHAPs_CE) using DLS and TEM, respectively. Overall, the present results showed that the synthesized nHAPs had a negative impact on the selected biochemical, cytotoxic, and genotoxic parameters, indicating that the evaluation of nHAP synthesized by this approach has a wide range of applications, especially as a nano-fertilizer.

纳米肥料是通过纳米技术方法制造的创新材料，由于其能在植物中快速吸收和控制养分分布，有可能取代传统肥料。在本研究中，采用石榴皮（PPE）和咖啡渣（CE）提取物的环保绿色合成方法，合成了含磷羟基磷灰石纳米颗粒（nHAP）作为新型磷纳米肥料。通过分析光合活性、碳水化合物水平、代谢产物等生化参数以及生菜中的生物相容性变化，对nHAP进行了物理化学表征和生物学评估。还基于对Vero细胞系的MTT试验研究了其与哺乳动物细胞的细胞相容性。使用动态光散射（DLS）和zeta电位分析来表征nHAP的大小和表面电荷，并使用扫描电子显微镜（SEM）和透射电子显微镜（TEM）来表征其形态。使用DLS和TEM分别发现nHAP具有不同形状，平均大小分别为229.6 nm、120.6 nm（nHAPs_PPE）和167.5 nm、153 nm（nHAPs_CE）。总体而言，目前的结果表明，合成的nHAP对所选的生化、细胞毒性和遗传毒性参数有负面影响，表明通过这种方法合成的nHAP评估具有广泛的应用，特别是作为纳米肥料。