Leong Shye Wei, Tan Shing Cheng, Norhayati Mohd Noor, Monif Mastura, Lee Si-Yuen
Department of Internal Medicine, School of Medical Sciences, Health Campus, Universiti Sains Malaysia, Kota Bharu 16150, Malaysia.
UKM Medical Molecular Biology Institute, Universiti Kebangsaan Malaysia, Kuala Lumpur 56000, Malaysia.
Cancers (Basel). 2022 Apr 26;14(9):2149. doi: 10.3390/cancers14092149.
Many medical applications have arisen from the technological advancement of three-dimensional (3D) bioprinting, including the printing of cancer models for better therapeutic practice whilst imitating the human system more accurately than animal and conventional in vitro systems. The objective of this systematic review is to comprehensively summarise information from existing studies on the effectiveness of bioinks in mimicking the tumour microenvironment of glioblastoma and their clinical value. Based on predetermined eligibility criteria, relevant studies were identified from PubMed, Medline Ovid, Web of Science, Scopus, and ScienceDirect databases. Nineteen articles fulfilled the inclusion criteria and were included in this study. Alginate hydrogels were the most widely used bioinks in bioprinting. The majority of research found that alginate bioinks had excellent biocompatibility and maintained high cell viability. Advanced structural design, as well as the use of multicomponent bioinks, recapitulated the native in vivo morphology more closely and resulted in bioprinted glioblastoma models with higher drug resistance. In addition, 3D cell cultures were superior to monolayer or two-dimensional (2D) cell cultures for the simulation of an optimal tumour microenvironment. To more precisely mimic the heterogenous niche of tumours, future research should focus on bioprinting multicellular and multicomponent tumour models that are suitable for drug screening.
三维（3D）生物打印的技术进步催生了许多医学应用，包括打印癌症模型以改善治疗实践，同时比动物和传统体外系统更准确地模拟人体系统。本系统综述的目的是全面总结现有研究中关于生物墨水在模拟胶质母细胞瘤肿瘤微环境方面的有效性及其临床价值的信息。根据预先确定的纳入标准，从PubMed、Medline Ovid、Web of Science、Scopus和ScienceDirect数据库中检索相关研究。19篇文章符合纳入标准并被纳入本研究。海藻酸盐水凝胶是生物打印中使用最广泛的生物墨水。大多数研究发现，海藻酸盐生物墨水具有优异的生物相容性，并能保持较高的细胞活力。先进的结构设计以及多组分生物墨水的使用，更接近地再现了体内天然形态，并产生了具有更高耐药性的生物打印胶质母细胞瘤模型。此外，在模拟最佳肿瘤微环境方面，3D细胞培养优于单层或二维（2D）细胞培养。为了更精确地模拟肿瘤的异质性生态位，未来的研究应专注于生物打印适合药物筛选的多细胞和多组分肿瘤模型。
