Gutierrez Charly, Pinson Xavier, Jarnouen Kathleen, Charpentier Marine, Pineau Raphael, Lallement Laëtitia, Pedeux Rémy
University Rennes, INSERM, OSS-UMR_S 1242, CLCC Eugène Marquis, 35042 Rennes, France.
University Rennes, CNRS, Inserm, Biosit UAR3480 US_S 018, MRic Core Facility, 35000 Rennes, France.
Cancers (Basel). 2022 Apr 26;14(9):2171. doi: 10.3390/cancers14092171.
Urine cytology is non-invasive, easy to collect, with medium sensitivity and a high specificity. It is an effective way to detect high-grade bladder cancer (BC), but it is less effective on low-grade BC because the rate of equivocal results is much higher. Recently, the fluorescent properties of plasma membranes of urothelial tumor cells (UTC) found in urine cytology have been shown to be useful in improving the early detection of BC. This phenomenon is called peri-membrane fluorescence (PMF). Based on previous studies that have identified the PMF on UTCs, the main objective was to characterize this phenomenon. For this study, a software was specially created to quantify the PMF of all tested cells and different treatments performed. PMF was not found to be a morphological and discriminating feature of UTCs, all cells in shape and not from urine show PMF. We were able to highlight the crucial role of plasma membrane integrity in the maintenance of PMF. Finally, it was found that the induction of a strong cellular stress induced a decrease in PMF, mimicking what was observed in non-tumor cells collected from urine. These results suggest that PMF is found in cells able to resist this stress, such as tumor cells.
尿液细胞学检查是非侵入性的，易于采集，具有中等敏感性和高特异性。它是检测高级别膀胱癌（BC）的有效方法，但对低级别BC的检测效果较差，因为结果不明确的比率要高得多。最近，尿液细胞学检查中发现的尿路上皮肿瘤细胞（UTC）质膜的荧光特性已被证明有助于提高BC的早期检测。这种现象被称为膜周荧光（PMF）。基于先前已在UTCs上鉴定出PMF的研究，主要目的是对这一现象进行表征。在本研究中，专门创建了一个软件来量化所有测试细胞的PMF以及所进行的不同处理。未发现PMF是UTCs的形态学和鉴别特征，所有形状的细胞以及非尿液来源的细胞均显示出PMF。我们能够突出质膜完整性在维持PMF中的关键作用。最后，发现强烈的细胞应激诱导会导致PMF降低，这与从尿液中收集的非肿瘤细胞中观察到的情况类似。这些结果表明，PMF存在于能够抵抗这种应激的细胞中，如肿瘤细胞。
