Nephrology Unit, Department of Internal Medicine and Pediatrics, Ghent University Hospital, 9000 Ghent, Belgium.
Burn Center, Ghent University Hospital, 9000 Ghent, Belgium.
Nutrients. 2022 Apr 27;14(9):1818. doi: 10.3390/nu14091818.
Advanced glycation end products (AGEs) are important in pathophysiology of type 2 diabetes mellitus (T2DM) and diabetic kidney disease (DKD). Dietary AGEs (dAGEs) contribute to the overall AGE pool in the body. Forty elderly T2DM patients with DKD were randomly allocated to a low-AGE ( = 20) or regular diabetic ( = 20) diet group. A three-day meal questionnaire was used to estimate average quantity of dAGEs. AGE accumulation was measured using skin autofluorescence and urine spectroscopy. sRAGE (soluble receptor AGE) was quantified using ELISA. After 8 weeks, the mean consumption of dAGEs was considerably reduced, both in the low-AGE diet ( = 0.004) and the control ( = 0.019) group. The expected urinary emission peak at 490 nm was shifted to 520 nm in some spectra. dAGEs did not correspond with urine AGE output. An AGE-limited diet for two months did not affect AGE content in skin and urine, or sRAGE concentration in the blood. The role of glycemia is likely to be greater than the impact of dAGE consumption. The unique observation of a fluorescence pattern at 520 nm warrants further examination, since it might point to genetic differences in AGE regulation, which could have clinical consequences, as AGE content depends on its formation and elimination.
糖基化终产物（AGEs）在 2 型糖尿病（T2DM）和糖尿病肾病（DKD）的病理生理学中很重要。饮食中的 AGEs（dAGEs）会增加体内 AGE 总量。40 名患有 DKD 的老年 T2DM 患者被随机分配到低 AGE（=20）或常规糖尿病（=20）饮食组。采用三天膳食问卷来估计 dAGEs 的平均摄入量。使用皮肤自发荧光和尿液光谱来测量 AGE 积累。使用 ELISA 定量 sRAGE（可溶性 AGE 受体）。8 周后，低 AGE 饮食（=0.004）和对照组（=0.019）中 dAGEs 的平均摄入量均明显减少。在一些光谱中，预期在 490nm 处的尿液 AGE 发射峰移至 520nm。dAGEs 与尿液 AGE 输出量不对应。两个月的 AGE 限制饮食并未影响皮肤和尿液中的 AGE 含量或血液中的 sRAGE 浓度。血糖的作用可能大于 dAGE 消耗的影响。在 520nm 处出现荧光模式的独特观察值得进一步研究，因为它可能指向 AGE 调节的遗传差异，这可能具有临床意义，因为 AGE 含量取决于其形成和消除。
