Laboratory of Basic Health Sciences, Department of Nursing, Faculty of Health Sciences, University of Peloponnese, 22100 Tripoli, Greece.
Haemodialysis Unit, General Hospital of Molaoi, 23052 Molaoi, Greece.
Int J Mol Sci. 2024 Aug 15;25(16):8893. doi: 10.3390/ijms25168893.
Exogenous and endogenous advanced glycation end products (AGEs) contribute to the pathogenesis and progression of renal disease. This is a one-month controlled dietary counseling trial that restricts nutritional AGEs in patients with end-stage renal disease (ESRD) undergoing haemodialysis ( = 22 participants in the intervention and = 20 participants in the control group). Haematological, biochemical markers, the soluble form of the receptor for AGEs (sRAGE), and carboxymethyl lysine (CML) were measured at baseline and at follow-up. Mononuclear cells were isolated and the protein expression of RAGE and the inflammatory marker COX-2 was measured using Western immunoblotting. The intervention group presented a lower increase in CML compared to the control group (12.39% median change in the intervention vs. 69.34% in the control group, = 0.013), while RAGE (% mean change -56.54 in the intervention vs. 46.51 in the control group, < 0.001) and COX-2 (% mean change -37.76 in the intervention vs. 0.27 in the control group, < 0.001) were reduced compared to the control group. sRAGE was reduced in both groups. In addition, HbA1c (at two months), total cholesterol, and triglycerides were reduced in the intervention versus the control group. The adoption of healthy cooking methods deserves further research as a possible way of modulating inflammatory markers in patients with CKD.
外源性和内源性晚期糖基化终产物（AGEs）导致肾脏疾病的发病机制和进展。这是一项为期一个月的对照饮食咨询试验，限制了终末期肾病（ESRD）患者接受血液透析时的营养性 AGEs（干预组 = 22 名参与者，对照组 = 20 名参与者）。在基线和随访时测量了血液学、生化标志物、AGEs 的可溶性受体（sRAGE）和羧甲基赖氨酸（CML）。分离单核细胞，并使用 Western 免疫印迹法测量 RAGE 和炎症标志物 COX-2 的蛋白表达。与对照组相比，干预组的 CML 增加幅度较低（干预组中位数变化 12.39%，对照组 69.34%， = 0.013），而 RAGE（%均值变化 -56.54 干预组与对照组 46.51%， < 0.001）和 COX-2（%均值变化 -37.76 干预组与对照组 0.27%， < 0.001）均低于对照组。两组的 sRAGE 均降低。此外，干预组的 HbA1c（在两个月时）、总胆固醇和甘油三酯均低于对照组。采用健康烹饪方法值得进一步研究，作为调节 CKD 患者炎症标志物的一种可能方法。
