College of Food Science and Nutritional Engineering, China Agriculture University, Beijing 100083, China.
Nutrients. 2022 May 7;14(9):1962. doi: 10.3390/nu14091962.
White-fleshed pitaya () and red-fleshed pitaya () are becoming increasingly popular because of their nutritional and medicinal benefits. However, in addition to their beneficial properties, allergy to pitaya fruits has occurred in daily life. In this study, we investigated the protein profile of pitaya fruit seeds and focused on the most reactive proteins against immunoglobulin E (IgE) in sera from allergic patients by immunoblotting. A protein band of approximately 20 kDa displayed a clear reaction with the serum IgE. The protein bands of interest were excised, in-gel digested, and analyzed using liquid chromatography-tandem mass spectrometry (LC-MS/MS), followed by data searching against a restricted database ( in UniProtKB) for protein identification. Immunoinformatic tools were used to predict protein allergenicity. The potential allergens included cupin_1 and heat shock protein 70 (HSP70) in white-fleshed pitaya seeds, and cupin_1, heat shock protein 70, and heat shock protein sti1-like in red-fleshed pitaya seeds are potential allergens. The expression of potential allergens was further verified at the transcriptional level in the species of and
白肉火龙果（）和红肉火龙果（）因其营养价值和药用价值而越来越受欢迎。然而，除了它们的有益特性外，日常生活中也发生了对火龙果果实的过敏反应。在这项研究中，我们研究了火龙果种子的蛋白质图谱，并通过免疫印迹法聚焦于过敏患者血清中对免疫球蛋白 E（IgE）反应最强烈的蛋白质。大约 20 kDa 的蛋白质带与血清 IgE 发生明显反应。感兴趣的蛋白质带被切下，胶内消化，并使用液相色谱-串联质谱（LC-MS/MS）进行分析，然后通过针对受限数据库（在 UniProtKB 中）进行数据搜索进行蛋白质鉴定。免疫信息学工具用于预测蛋白质的变应原性。潜在的过敏原包括白肉火龙果种子中的 cupin_1 和热休克蛋白 70（HSP70），以及红肉火龙果种子中的 cupin_1、热休克蛋白 70 和热休克蛋白 sti1 样蛋白。潜在过敏原的表达在转录水平上进一步在 和 的种属中得到了验证。
