Qin Qiulin, Li Jingling, Zeng Siyuan, Xu Yiceng, Han Fang, Yu Jie
College of Horticulture and Landscape Architecture, Southwest University, No.2 Tiansheng Road, Beibei District, Chongqing, 400716 China.
Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education, Chongqing, 400716 China.
Physiol Mol Biol Plants. 2022 Jan;28(1):123-137. doi: 10.1007/s12298-021-01121-z. Epub 2022 Jan 11.
is a genus of perennial shrub from the family Cactaceae, and some of them play an important role in the food industry, pharmaceuticals, cosmetics and medicine. To date, there are few reports on plastomes, which limits our understanding of this genus. Here, we have reported the complete plastomes of four species (, , and and carried out a comprehensive comparative analysis. All four plastomes have a typical quartile structure. The plastome size ranged from 133,146 to 134,450 bp, and contained 104 unique genes, including 30 tRNA genes, 4 rRNA genes and 70 protein-coding genes. Comparative analysis showed that there were massive losses of genes in . Besides, we observed the inverted repeat regions had undergone a dramatic expansion and formed a previously unreported small single copy/inverted repeat border in the intron region of the F gene. Furthermore, we identified 6 hypervariable regions (F-GAA-L, 1, D, P-S-GCU, P-T-CGU and 22-19) that could be used as potential DNA barcodes for the identification of species. Our study enriches the plastome in the family Cactaceae, and provides the basis for the reconstruction of phylogenetic relationships.
The online version contains supplementary material available at 10.1007/s12298-021-01121-z.
是仙人掌科多年生灌木属，其中一些在食品工业、制药、化妆品和医学中发挥着重要作用。迄今为止，关于质体基因组的报道很少，这限制了我们对该属的了解。在这里，我们报道了四个物种（、、和）的完整质体基因组，并进行了全面的比较分析。所有四个质体基因组都具有典型的四分体结构。质体基因组大小在133,146至134,450碱基对之间，包含104个独特基因，包括30个tRNA基因、4个rRNA基因和70个蛋白质编码基因。比较分析表明，中存在大量基因丢失。此外，我们观察到反向重复区域经历了显著扩展，并在F基因内含子区域形成了一个以前未报道的小单拷贝/反向重复边界。此外，我们鉴定出6个高变区域（F-GAA-L、1、D、P-S-GCU、P-T-CGU和22-19），可作为鉴定物种的潜在DNA条形码。我们的研究丰富了仙人掌科的质体基因组，并为系统发育关系的重建提供了基础。
在线版本包含可在10.1007/s12298-021-01121-z获取的补充材料。
