Institute of Agrophysics, Polish Academy of Sciences, Doświadczalna 4, 20-290 Lublin, Poland.
Department of Biochemistry, Institute of Soil Science and Plant Cultivation, State Research Institute, 24-100 Puławy, Poland.
Molecules. 2022 Apr 19;27(9):2624. doi: 10.3390/molecules27092624.
The goal of this work is to evaluate the hop stems, a byproduct of hop cones production, as a potential source of cellulose. Hop stems contain up to 29% of cellulose. The cellulose isolation was conducted through the thermochemical treatment. After high-speed blending, the cellulose was characterized by 67% of crystallinity degree obtained from X-ray diffraction and median diameter of 6.7 nm obtained from atomic force microscopy imaging. The high-intensity ultrasonication (HIUS) was applied to reach further disintegration of cellulose fibers. The longer HIUS treatment resulted in decrease in crystallinity degree even up to 60% and decrease in the fiber diameter up to 4 nm. The Fourier transform infrared spectroscopy (FTIR) spectra showed that HIUS treatment led to changes in intermolecular hydrogen bonds. The stability of cellulose dispersions versus length of HIUS treatment was monitored over 14 days with back dynamic light scattering and laser Doppler electrophoresis methods. Obtained results are evidence that the hop stems are a potential source of cellulose and that it is possible to obtain stable dispersions after HIUS treatment. This was the first time that the properties of hop cellulose have been described so extensively and in detail after the use of HIUS treatment.
这项工作的目的是评估啤酒花茎，即啤酒花球果生产的副产品，作为纤维素的潜在来源。啤酒花茎中含有高达 29%的纤维素。通过热化学处理进行纤维素分离。高速混合后，通过 X 射线衍射获得结晶度为 67%的纤维素，并通过原子力显微镜成像获得中值直径为 6.7nm 的纤维素。高强度超声处理（HIUS）用于实现纤维素纤维的进一步细化。较长时间的 HIUS 处理会导致结晶度降低，甚至降低 60%，纤维直径降低 4nm。傅里叶变换红外光谱（FTIR）谱表明 HIUS 处理导致分子间氢键发生变化。通过背向动态光散射和激光多普勒电泳方法监测纤维素分散体在 HIUS 处理 14 天内的稳定性。结果表明，啤酒花茎是纤维素的潜在来源，并且在 HIUS 处理后可以获得稳定的分散体。这是首次如此广泛和详细地描述使用 HIUS 处理后的啤酒花纤维素的性质。
