Chemistry Department, University of Sciences, Hue University, Hue 530000, Vietnam.
Faculty of Environmental Sciences, Saigon University, Ho Chi Minh City 700000, Vietnam.
Molecules. 2022 Apr 20;27(9):2657. doi: 10.3390/molecules27092657.
In this paper, the classical least-squares (CLS) method with molecular absorption spectrophotometric measurement was used to determine simultaneously paracetamol (PAR), ibuprofen (IBU), and caffeine (CAF) in tablets. The absorbance spectra of the standard solutions and samples were measured over a wavelength from 220 to 300 nm with a 0.5 nm step. The concentration of PAR, IBU, and CAF in the sample solutions was calculated by using Visual Basic for Applications (VBA) and a program called CLS-Excel written in Microsoft Excel 2016. The method and the CLS-Excel program were tested on mixed standard laboratory samples with different PAR, IBU, and CAF concentration ratios, and they showed only small errors and a satisfying repeatability. An analytical procedure for tablets containing PAR, IBU, and CAF was developed. The reliability of the procedure was proved via the recovery and repeatability of the analysis results with an actual tablet sample and by comparing the mean contents of active substances in the tablets obtained from the analytical procedure with the HPLC method. The procedure is simple with a reduced cost compared with the HPLC standard method.
本文采用经典最小二乘法（CLS）与分子吸收光谱光度法，同时测定片剂中的对乙酰氨基酚（PAR）、布洛芬（IBU）和咖啡因（CAF）。在波长 220 至 300nm 范围内，以 0.5nm 为步长，测量标准溶液和样品的吸光度光谱。使用 Visual Basic for Applications（VBA）和在 Microsoft Excel 2016 中编写的名为 CLS-Excel 的程序，通过计算得出样品溶液中 PAR、IBU 和 CAF 的浓度。对不同 PAR、IBU 和 CAF 浓度比的混合标准实验室样品进行了方法和 CLS-Excel 程序的测试，结果表明误差较小，重复性较好。开发了一种含有 PAR、IBU 和 CAF 的片剂分析程序。通过对实际片剂样品的分析结果进行回收率和重复性验证，以及将通过分析程序获得的片剂中活性物质的平均含量与 HPLC 法进行比较，证明了该程序的可靠性。与 HPLC 标准方法相比，该程序简单，成本降低。
