School of Pharmacy, Qiqihar Medical University, Qiqihar 161006, China.
Qiqihar Institute for Food and Drug Control, Qiqihar 161006, China.
Molecules. 2022 Apr 20;27(9):2650. doi: 10.3390/molecules27092650.
Estrogens added illegally to dietary supplements are hazardous to human health. Traditional detection and analysis methods have many limitations, and we have developed an assay that combines thin-layer chromatography with Raman imaging microscopy (TLC-RIM). The five estrogens (estrone, estradiol, estriol, ethinyl estradiol, and diethylstilbestrol) were initially separated by TLC, then detected by area scanning Raman imaging with a 532 nm laser under a microscope. Raman spectra were obtained for each estrogen, which were used for detecting estrogen illegally added to botanical dietary supplements. The LOD of each estrogen was 0.4, 1.0, 0.8, 0.2, and 0.2 mg/mL, respectively. The matrix in the real sample did not interfere with the detection of estrogens. The method was fast, sensitive, stable, specific, and reliable.
雌激素被非法添加到膳食补充剂中会对人类健康造成危害。传统的检测和分析方法有许多局限性，我们开发了一种结合薄层色谱和拉曼成像显微镜（TLC-RIM）的分析方法。五种雌激素（雌酮、雌二醇、雌三醇、乙炔雌二醇和己烯雌酚）最初通过 TLC 分离，然后用显微镜下 532nm 激光进行面积扫描拉曼成像检测。对每种雌激素进行拉曼光谱分析，用于检测添加到植物性膳食补充剂中的雌激素。每种雌激素的 LOD 分别为 0.4、1.0、0.8、0.2 和 0.2mg/mL。实际样品中的基质不会干扰雌激素的检测。该方法快速、灵敏、稳定、特异、可靠。
