School of Pharmacy, Shenyang Pharmaceutical University, No. 26 Huatuo Rd, High & New Tech Development Zone, 117004, Benxi, Liaoning, P. R. China.
Mikrochim Acta. 2024 Jul 22;191(8):474. doi: 10.1007/s00604-024-06546-3.
A novel magnetic dispersive solid phase extraction (MDSPE) procedure based on the deep eutectic solvent (DES) modified magnetic graphene oxide/metal organic frameworks nanocomposites (MGO@ZIF-8@DES) was established and used for the efficient enrichment of estradiol, estrone, and diethylstilbestrol in cosmetics (toner, lotion, and cream) for the first time. Then, the three estrogens were separated and determined by UHPLC-UV analysis method. In order to study the features and morphology of the synthesized adsorbents, various techniques such as FT-IR, SEM, and VSM measurements were executed. The MGO@ZIF-8@DES nanocomposites combine the advantages of high adsorption capacity, adequate stability in aqueous solution, and convenient separation from the sample solution. To achieve high extraction recoveries, the Box-Behnken design and single factor experiment were applied in the experimental design. Under the optimum conditions, the method detection limits for three estrogens were 20-30 ng g. This approach showed a good correlation coefficient (r more than 0.9998) and reasonable linearity in the range 70-10000 ng g. The relative standard deviations for intra-day and inter-day were beneath 7.5% and 8.9%, respectively. The developed MDSPE-UHPLC-UV method was successfully used to determine three estrogens in cosmetics, and acceptable recoveries in the intervals of 83.5-95.9% were obtained. Finally, three estrogens were not detected in some cosmetic samples. In addition, the Complex GAPI tool was used to evaluate the greenness of the developed pretreatment method. The developed MDSPE-UHPLC-UV method is sensitive, accurate, rapid, and eco-friendly, which provides a promising strategy for determining hormones in different complex samples.
一种基于深共晶溶剂（DES）修饰的磁性氧化石墨烯/金属有机骨架纳米复合材料（MGO@ZIF-8@DES）的新型磁性分散固相萃取（MDSPE）程序被建立并首次用于高效富集化妆品（粉饼、乳液和面霜）中的雌二醇、雌酮和己烯雌酚。然后，通过 UHPLC-UV 分析方法分离和测定这三种雌激素。为了研究合成吸附剂的特征和形态，采用了 FT-IR、SEM 和 VSM 等多种技术进行了测量。MGO@ZIF-8@DES 纳米复合材料结合了高吸附容量、在水溶液中足够稳定和便于从样品溶液中分离的优点。为了实现高萃取回收率，在实验设计中应用了 Box-Behnken 设计和单因素实验。在最佳条件下，三种雌激素的方法检测限为 20-30ng g。该方法在 70-10000ng g 的范围内表现出良好的相关性（r 大于 0.9998）和合理的线性。日内和日间相对标准偏差分别低于 7.5%和 8.9%。所建立的 MDSPE-UHPLC-UV 方法成功地用于测定化妆品中的三种雌激素，回收率在 83.5-95.9%的范围内。最后，在一些化妆品样品中未检测到三种雌激素。此外，还使用了 Complex GAPI 工具来评估所开发预处理方法的绿色度。所开发的 MDSPE-UHPLC-UV 方法灵敏、准确、快速且环保，为测定不同复杂样品中的激素提供了有前景的策略。
