Animal Physiology and Phytopharmacology Laboratory, University of Dschang, Cameroon.
BMC Complement Altern Med. 2013 Jan 7;13:4. doi: 10.1186/1472-6882-13-4.
Mondia whitei and Guibourtia tessmannii are used in Cameroon traditional medicine as aphrodisiacs. The present study was undertaken to evaluate the pro-ejaculatory effects of the aqueous and organic solvent extracts of these plants in spinal male rats.
In spinal cord transected and urethane-anesthetized rats, two electrodes where inserted into the bulbospongiosus muscles and the ejaculatory motor pattern was recorded on a polygraph after urethral and penile stimulations, intravenous injection of saline (0.1 ml/100 g), dopamine (0.1 μM/kg), aqueous and organic solvent plant extracts (20 mg/kg).
In all spinal rats, urethral and penile stimulations always induced the ejaculatory motor pattern. Aqueous or hexane extract of Mondia whitei (20 mg/kg) prevented the expression of the ejaculatory motor pattern. The pro-ejaculatory effects of dopamine (0.1 μM/kg) were not abolished in spinal rats pre-treated with Mondia whitei extracts. Aqueous and methanolic stem bark extracts of Guibourtia tessmannii (20 mg/kg) induced fictive ejaculation characterized by rhythmic contractions of the bulbospongiosus muscles followed sometimes with expulsion of seminal plugs. In rats pre-treated with haloperidol (0.26 μM/kg), no ejaculatory motor pattern was recorded after intravenous injection of Guibourtia tessmannii extracts (20 mg/kg).
These results show that Mondia whitei possesses preventive effects on the expression of fictive ejaculation in spinal male rats, which is not mediated through dopaminergic pathway; on the contrary, the pro-ejaculatory activities of Guibourtia tessmannii require the integrity of dopaminergic system to exert its effects. The present findings further justify the ethno-medicinal claims of Mondia whitei and Guibourtia tessmannii.
在喀麦隆传统医学中，白叶蒙迪亚和特氏古夷苏木被用作春药。本研究旨在评估这些植物的水提物和有机溶剂提取物对脊髓雄性大鼠射精的促进作用。
在脊髓横断和氨基甲酸乙酯麻醉的大鼠中，将两个电极插入球海绵体肌，在尿道和阴茎刺激后，通过多导记录仪记录射精运动模式，静脉注射生理盐水（0.1ml/100g）、多巴胺（0.1μM/kg）、植物的水提物和有机溶剂提取物（20mg/kg）。
在所有脊髓大鼠中，尿道和阴茎刺激总是引起射精运动模式。白叶蒙迪亚的水提物或己烷提取物（20mg/kg）可阻止射精运动模式的表达。预先用白叶蒙迪亚提取物处理的脊髓大鼠中，多巴胺（0.1μM/kg）的促射精作用并未被消除。特氏古夷苏木的水提物和甲醇树皮提取物（20mg/kg）可引起虚构的射精，表现为球海绵体肌有节奏的收缩，有时伴随着精液栓的排出。在预先给予氟哌啶醇（0.26μM/kg）的大鼠中，静脉注射特氏古夷苏木提取物（20mg/kg）后，没有记录到射精运动模式。
这些结果表明，白叶蒙迪亚对脊髓雄性大鼠虚构射精的表达具有预防作用，这不是通过多巴胺能途径介导的；相反，特氏古夷苏木的促射精活性需要多巴胺能系统的完整性才能发挥作用。本研究结果进一步证实了白叶蒙迪亚和特氏古夷苏木的民族医学用途。
