Bukhari Syed Nasir Abbas, Ali Arshad, Hussain Muhammad Ajaz, Tayyab Muhammad, Alotaibi Nasser F, Elsherif Mervat A, Junaid Kashaf, Ejaz Hasan
Department of Pharmaceutical Chemistry, College of Pharmacy, Jouf University, Sakaka 72388, Saudi Arabia.
Institute of Chemistry, University of Sargodha, Sargodha 40100, Pakistan.
Polymers (Basel). 2022 May 6;14(9):1904. doi: 10.3390/polym14091904.
seed mucilage (MPM) is composed of glucuronoxylan, which is a swellable, pH-responsive and non-toxic biomaterial. Herein, we aimed to extract MPM from seeds (MP seeds) to ascertain optimization of extraction conditions to get highest yield by response surface methodology, via Box-Behnken design (RSM-BBD). MPM was extracted from MP seeds by a hot water extraction method. The effects of four different parameters on the extraction yield of MPM were evaluated: pH of the extraction medium (1-10), seed/water contact time (1-12 h), the temperature of extraction medium (30-90 °C), and seed/water ratio (1:5-1:35 /). The maximum yield of MPM obtained by Design-Expert software was 10.66% (10.66 g/100 g) at pH 7, seed/water contact time of 6 h, extraction temperature of 50 °C, and seed/water ratio of 1:20 /. The values of ANOVA were found to be less than 0.0001, which indicated that the extraction yield of MPM was significantly affected by all the study parameters. The results revealed that pH and extraction temperature were the most significant factors affecting the yield of MPM. MPM in compressed tablet form showed pH-responsive on-off switching behavior at pH 7.4 and 1.2 in a reversible manner. MPM in compressed tablet form sustained the release of itopride for 16 h following a super case-II transport mechanism and zero-order release kinetics.
种子黏液（MPM）由葡糖醛酸木聚糖组成，它是一种可膨胀、对pH有响应且无毒的生物材料。在此，我们旨在从种子（MP种子）中提取MPM，通过响应面法（RSM - BBD），即Box - Behnken设计，确定提取条件的优化以获得最高产量。采用热水提取法从MP种子中提取MPM。评估了四个不同参数对MPM提取率的影响：提取介质的pH值（1 - 10）、种子/水接触时间（1 - 12小时）、提取介质温度（30 - 90℃）以及种子/水比例（1:5 - 1:35 /）。通过Design - Expert软件获得的MPM最大产量在pH值为7、种子/水接触时间为6小时、提取温度为50℃以及种子/水比例为1:20 /时为10.66%（10.66克/100克）。方差分析（ANOVA）值小于0.0001，这表明所有研究参数对MPM的提取率均有显著影响。结果表明，pH值和提取温度是影响MPM产量的最显著因素。压缩片剂形式的MPM在pH值为7.4和1.2时以可逆方式表现出pH响应的开关行为。压缩片剂形式的MPM遵循超Ⅱ型转运机制和零级释放动力学，使伊托必利持续释放16小时。
