Liu Yang, Yuan Yichao, Jiang Zhuoke, Jin Songheng
Jiyang College, Zhejiang A&F University, Zhuji 311800, China.
Zhejiang Provincial Key Laboratory of Resources Protection and Innovation of Traditional Chinese Medicine, Zhejiang A&F University, Hangzhou 311300, China.
Plants (Basel). 2022 Apr 25;11(9):1157. doi: 10.3390/plants11091157.
is commonly used to treat diabetes in China. However, the natural habitats of are typically affected by salt stress. Previous studies showed that nitric oxide (NO) level was related to salt tolerance of , and its synthesis was induced by exogenous hydrogen sulfide. However, the effects of different NO donors in alleviating the negative effect of salt stress are still unclear. In the present study, seedlings pretreated with three NO donors (S-nitroso-N-acetylpenicillamine, SNAP and S-nitrosoglutathione, GSNO and sodium nitroprusside, SNP) were exposed to salt stress, and then, the total biomass, chlorophyll fluorescence parameters, NO and glutathione levels, oxidative damage, and antioxidant enzyme activities were investigated. The results showed that pretreatment of NO donors maintained chlorophyll fluorescence and attenuated the loss of plant biomass under salt stress, and the best performance was observed in under SNP treatment. We also found that pretreatment of NO donors further increased the endogenous NO content and nitrate reductase (NR) activity compared with salt treatment. Moreover, pretreatment with NO donors, especially SNP, alleviated salt-induced oxidative damage, as indicated by lowered lipid peroxidation, through an enhanced antioxidant system including glutathione accumulation and increased antioxidant enzyme activities. The supply of NO donors is an interesting strategy for alleviating the negative effect of salt on . Our data provide new evidence contributing to the current understanding of NO-induced salt stress tolerance.
在中国常用于治疗糖尿病。然而，其天然栖息地通常受到盐胁迫的影响。先前的研究表明，一氧化氮（NO）水平与耐盐性有关，并且其合成由外源性硫化氢诱导。然而，不同NO供体在减轻盐胁迫负面影响方面的作用仍不清楚。在本研究中，用三种NO供体（S-亚硝基-N-乙酰青霉胺、SNAP和S-亚硝基谷胱甘肽、GSNO以及硝普钠、SNP）预处理的幼苗暴露于盐胁迫下，然后研究了总生物量、叶绿素荧光参数、NO和谷胱甘肽水平、氧化损伤以及抗氧化酶活性。结果表明，NO供体预处理在盐胁迫下维持了叶绿素荧光并减轻了植物生物量的损失，在SNP处理下表现最佳。我们还发现，与盐处理相比，NO供体预处理进一步增加了内源性NO含量和硝酸还原酶（NR）活性。此外，NO供体预处理，尤其是SNP，通过增强包括谷胱甘肽积累和抗氧化酶活性增加的抗氧化系统，减轻了盐诱导的氧化损伤，如脂质过氧化降低所示。提供NO供体是减轻盐对负面影响的一种有趣策略。我们的数据为当前对NO诱导的盐胁迫耐受性的理解提供了新的证据。
