昆斯精油的化学成分、对映体含量、抗氧化及抗胆碱酯酶活性
Chemical Constituents, Enantiomer Content, Antioxidant and Anticholinesterase Activities of Kunth Essential Oil.
作者信息
Aguilar Gabriela, Calva James, Cartuche Luis, Salinas Melissa, Armijos Chabaco
机构信息
Departamento de Química y Ciencias Exactas, Universidad Tecnica Particular de Loja, Loja 1101608, Ecuador.
出版信息
Plants (Basel). 2023 May 30;12(11):2155. doi: 10.3390/plants12112155.
The study of the essential oil (EO) from aerial parts (stems and leaves) of Kunth (Valerianaceae), collected from the Saraguro community in the southern region of Ecuador, was analyzed for the first time. A total of 62 compounds were identified in EO by GC-FID and GC-MS on nonpolar DB-5ms and polar HP-INNOWax columns. The most abundant components (>5%) detected on DB-5ms and polar HP-INNOWax columns were -gurjunene (11.98, 12.74%), germacrene D (11.47, 14.93%), -caryophyllene (7.05, 7.78%), and -copaene (6.76, 6.91%), respectively. In addition, the enantioselective analysis, carried out on a chiral column, showed (+)-α-pinene and (R)-(+)-germacrene as enantiomerically pure compounds (enantiomeric excess = 100%). The antioxidant activity was high for the radicals ABTS (SC50 = 41.82 µg/mL) and DPPH (SC50 = 89.60 µg/mL), and finally, the EO was shown to be inactive to the enzyme acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE), as both values were >250 µg/mL.
首次对采自厄瓜多尔南部萨拉古罗社区的昆特（缬草科）地上部分（茎和叶）的精油（EO）进行了研究。通过气相色谱 - 氢火焰离子化检测器（GC - FID）和气相色谱 - 质谱联用仪（GC - MS）在非极性DB - 5ms柱和极性HP - INNOWax柱上对EO中的62种化合物进行了鉴定。在DB - 5ms柱和极性HP - INNOWax柱上检测到的最丰富成分（>5%）分别是 - 古芸烯（11.98，12.74%）、杜松烯D（11.47，14.93%）、 - 石竹烯（7.05，7.78%）和 - 古巴烯（6.76，6.91%）。此外，在手性柱上进行的对映体选择性分析表明，（+）-α-蒎烯和（R）-（+）-杜松烯是对映体纯化合物（对映体过量 = 100%）。对ABTS自由基（SC50 = 41.82 µg/mL）和DPPH自由基（SC50 = 89.60 µg/mL）的抗氧化活性较高，最后，该EO对乙酰胆碱酯酶（AChE）和丁酰胆碱酯酶（BuChE）均无活性，因为两者的值均>250 µg/mL。
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