College of Agronomy, Hunan Agricultural University, Changsha, 410128, China; Guangdong Key Laboratory for Crop Germplasm Resources Preservation and Utilization, Agro-biological Gene Research Center, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.
Guangdong Key Laboratory for Crop Germplasm Resources Preservation and Utilization, Agro-biological Gene Research Center, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.
Plant Physiol Biochem. 2022 Jul 15;183:85-95. doi: 10.1016/j.plaphy.2022.04.028. Epub 2022 Apr 30.
The glyoxalase pathway plays a vital role in the chemical detoxification of methylglyoxal (MG) in biological systems. Our previous study suggested that OsGLYI3 may be effective in seed natural aging. In this study, the rice OsGLYI3 gene was cloned and characterized as specifically expressed in the seed. The accelerated aging (AA) treatment results indicated significant roles of OsGLYI3 in seed longevity and vigor, as the seeds of the transgenic lines with overexpressed and knocked-out OsGLYI3 exhibited higher and lower germination, respectively. The AA treatment also increased the superoxide dismutase (SOD) activity in the overexpressed transgenic seeds compared to the wild-type seeds yet lowered the SOD activity in the CRISPR/Cas9-derived transgenic rice lines. Rice OsGLYI3 was markedly upregulated in response to NaCl induced stress conditions. Compared to wild-type plants, overexpressed transgenic rice lines exhibited increased GLYI activity, decreased MG levels and improved salt stress tolerance, while CRISPR/Cas9 knockout transgenic rice lines showed decreased glyoxalase I activity, increased MG levels, and greater sensitivity to stress treatments with NaCl. Collectively, our results confirmed for the first time that OsGLYI3 is specifically expressed in rice seeds and contributes to seed longevity and salt stress tolerance.
糖氧还蛋白途径在生物系统中对甲基乙二醛 (MG) 的化学解毒中起着至关重要的作用。我们之前的研究表明，OsGLYI3 可能在种子自然衰老中有效。在这项研究中，克隆并表征了水稻 OsGLYI3 基因，该基因特异性表达于种子中。加速老化 (AA) 处理结果表明 OsGLYI3 在种子寿命和活力中起着重要作用，因为过表达和敲除 OsGLYI3 的转基因系的种子表现出更高和更低的发芽率。AA 处理还增加了超氧化物歧化酶 (SOD) 活性在过表达转基因种子中比野生型种子高，但降低了 CRISPR/Cas9 衍生的转基因水稻系中的 SOD 活性。水稻 OsGLYI3 对 NaCl 诱导的胁迫条件明显上调。与野生型植物相比，过表达转基因水稻系表现出增强的 GLYI 活性、降低的 MG 水平和提高的耐盐性，而 CRISPR/Cas9 敲除转基因水稻系表现出降低的糖氧还蛋白 I 活性、增加的 MG 水平和对 NaCl 胁迫处理更敏感。总之，我们的研究结果首次证实，OsGLYI3 特异性表达于水稻种子，并有助于种子寿命和耐盐性。
