Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, Beijing 100083, China; National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing 100083, China; Key Laboratory for Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China.
National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, No. 35, Qinghua East Road, Haidian District, Beijing 100083, China.
Genomics. 2022 Jul;114(4):110385. doi: 10.1016/j.ygeno.2022.110385. Epub 2022 May 13.
Photosynthesis plays vital role in plant growth and development throughout its life, and it is influenced by environmental signals and circadian rhythms. We analyzed the transcriptome landscape of the two poplars progeny with contrasting photosynthesis rates at three times point (ZT4, ZT16, ZT22), constructed gene regulatory network that related to circadian rhythm and photosynthesis. We suggest that the differences in photosynthetic rate between the progenies may originate from plant endogenous circadian oscillators prepare poplar plants for photosynthesis by regulating photosynthesis-associated nuclear genes and carotenoid metabolism genes before dawn, genes associated with plant hormone signal transduction and transcription factor increase leaf size and stomatal movement, the influence of other core regulatory factors on chlorophyll accumulation. Furthermore, overexpression of candidate regulatory gene, AP3 (Potri.007G017000), induced leaf senescence and reduced the content of chlorophyll. These results demonstrated that many potential key regulators are integrated closely with chlorophyll content and photosynthesis.
光合作用在植物的整个生命周期中对其生长和发育起着至关重要的作用，并且受到环境信号和昼夜节律的影响。我们在三个时间点（ZT4、ZT16、ZT22）分析了具有不同光合作用速率的两个杨树后代的转录组图谱，构建了与昼夜节律和光合作用相关的基因调控网络。我们认为，后代之间光合作用速率的差异可能源于植物内源性生物钟振荡器通过在黎明前调节光合作用相关核基因和类胡萝卜素代谢基因，为杨树植物的光合作用做准备，与植物激素信号转导和转录因子相关的基因增加叶片大小和气孔运动，其他核心调节因子对叶绿素积累的影响。此外，候选调节基因 AP3（Potri.007G017000）的过表达诱导了叶片衰老并降低了叶绿素含量。这些结果表明，许多潜在的关键调节因子与叶绿素含量和光合作用密切相关。
