Univ Lyon, INSA Lyon, CNRS, IMP UMR 5223, F-69622, Villeurbanne, France.
Center for Sustainable Materials, School of Materials Science and Engineering, Nanyang Technological University (NTU), 50 Nanyang Drive, Singapore 637553, Singapore.
ACS Macro Lett. 2021 May 18;10(5):628-634. doi: 10.1021/acsmacrolett.1c00171. Epub 2021 May 3.
Herein, we report on the precise design of a modular fusion protein amenable to the construction of nanocapsules by nanoprecipitation. The central squid suckerin-derived peptide block provides structural stability, whereas both termini from spider silk fibroins make the protein highly soluble at physiological pH, a critical requirement for the nanoprecipitation process. With this design, nanocapsules consisting of fusion protein shells and oily cores with sizes in the range of 190-250 nm are built in a straightforward manner.
在此，我们报告了一种模块化融合蛋白的精确设计，该融合蛋白可通过纳米沉淀法构建纳米胶囊。中心来自鱿鱼吸盘的肽段提供结构稳定性，而两端来自蜘蛛丝丝蛋白使该蛋白在生理 pH 值下高度溶解，这是纳米沉淀过程的关键要求。通过这种设计，可以以简单的方式构建融合蛋白壳和油芯尺寸在 190-250nm 范围内的纳米胶囊。
