BACKGROUND

Fibrillin (FBN) proteins are widely distributed in the photosynthetic organs. The members of gene family play important roles in plant growth and development, and response to hormone and stresses. Tomato is a vegetable crop with significantly economic value and model plant commonly used in research. However, the FBN family has not been systematical studied in tomato.

METHODS

In this study, 14 genes were identified in tomato genome by Pfam and Hmmer 3.0 software. ExPASy, MEGA 6.0, MEME, GSDS, TBtools, PlantCARE and so on were used for physical and chemical properties analysis, phylogenetic analysis, gene structure and conserved motifs analysis, collinearity analysis and cis-acting element analysis of family genes in tomato. Expression characteristics of in different tissues, fruit shape near isogenic lines (NILs), DC3000 and ABA treatments were analyzed based on transcriptome data and quantitative Real-time qPCR (qRT-PCR) analysis.

RESULTS

The family was divided into 11 subgroups. There were 8 homologous gene pairs between tomato and . All the members of SlFBN family contained PAP conserved domain, but their gene structure and conserved motifs showed apparent differences. The cis-acting elements of light and hormone (especially ethylene, methyl jasmonate (MeJA) and abscisic acid (ABA)) were widely distributed in the promoter regions. The expression analysis found that most of were predominantly expressed in leaves of Heinz and LA1589, and showed higher expressions in mature or senescent leaves than in young leaves. Expression analysis of different tissues and fruit shape NILs indicated , and might play important roles during tomato fruit differentiation. All of the responded to DC3000 and ABA treatments. The results of this study contribute to exploring the functions and molecular mechanisms of in leaf development, fruit differentiation, stress and hormone responses.