Gansu Agricultural University, College of Horticulture, Lanzhou, Gansu, China.
PeerJ. 2022 Feb 10;10:e12943. doi: 10.7717/peerj.12943. eCollection 2022.
Serine hydroxymethyltransferase (SHMT) is one of the most important enzyme families in one-carbon metabolic pathway and photorespiration within plant cells. Recently studies reported the active roles of plant SHMTs in defending abiotic stresses. However, genome-scale analysis of SHMT in tomato is currently unknown. In this study, seven genes were identified in the tomato genome using a genome-wide search approach. In addition, their physicochemical properties, protein secondary structure, subcellular localization, gene structure, conserved motifs, phylogenetic and collinear relationships were analyzed. Our results demonstrated that tomato SHMT members were divided into two group and four subgroups, and they were conserved with the orthologs of other plants. Analysis of -acting elements showed that each of the genes contained different kinds of hormones and stress-related -acting elements in their promoter regions. Finally, qRT-PCR analysis indicated that s were expressed at different levels in different tissues, and they responded to UV, cold, heat, NaCl, HO, ABA and PEG treatments. These results provided definite evidence that s might involve in growth, development and stress responses in tomato, which laid a foundation for future functional studies of s.
丝氨酸羟甲基转移酶（SHMT）是植物细胞内一碳代谢途径和光呼吸中最重要的酶家族之一。最近的研究报道了植物 SHMT 在抵御非生物胁迫中的积极作用。然而，目前尚不清楚番茄 SHMT 的全基因组分析情况。在本研究中，我们使用全基因组搜索方法在番茄基因组中鉴定出了 7 个基因。此外，还对它们的理化性质、蛋白质二级结构、亚细胞定位、基因结构、保守基序、系统发育和共线性关系进行了分析。结果表明，番茄 SHMT 成员分为 2 组和 4 个亚组，与其他植物的同源物具有保守性。顺式作用元件分析表明，每个基因的启动子区域都含有不同种类的激素和与应激相关的顺式作用元件。最后，qRT-PCR 分析表明，s 在不同组织中的表达水平不同，它们对 UV、冷、热、NaCl、HO、ABA 和 PEG 处理有响应。这些结果为 s 可能参与番茄的生长、发育和应激反应提供了明确的证据，为今后 s 的功能研究奠定了基础。
