NEOGEN Corporation, 620 Lesher Pl, Lansing, MI 48912, USA.
TEQ Analytical Laboratories, 12635 E. Montview Blvd #175, Aurora, CO 80045, USA.
J AOAC Int. 2022 Sep 6;105(5):1423-1427. doi: 10.1093/jaoacint/qsac057.
The Soleris® Coliform Vial is a growth-based, automated method for detection of coliform bacteria in foods and other consumer products such as nutraceuticals and cosmetics. The method was granted AOAC Performance Tested MethodSM certification for select foods after successful completion of a validation study.
The objective of the current study was to validate the Soleris coliform method for use with dried cannabis flower (>0.3% delta 9-tetrahydrocannabinol).
A comparative matrix study was performed in which naturally contaminated dried cannabis flower was tested with the Soleris coliform method and with the U.S. Food and Drug Administration Bacteriological Analytical Manual solid medium method. Multiple lots of dried cannabis flower were obtained, pre-screened for coliforms, and blended to produce test materials at four different contamination levels ranging from 4.5 to 1600 CFU/g. Each material was tested at three different Soleris detection threshold levels determined by the dilution used to inoculate the Soleris vials. Probability of detection analysis was performed to determine if differences in the number of positive results obtained with the two methods were significant.
For all four dried cannabis flower materials, at all three Soleris test thresholds, there were no significant differences in the number of positive results obtained with the Soleris and cultural plating methods as determined by probability of detection analysis at P < 0.05.
The Soleris coliform test is an accurate method for detection of coliform bacteria in dried cannabis flower.
The Soleris coliform method provides cannabis industry QC personnel with an effective method for analysis of dried cannabis flower and produces results in 18-24 h.
Soleris®大肠菌群检测管是一种基于生长的自动化方法，用于检测食品和其他消费品（如营养保健品和化妆品）中的大肠菌群。该方法在成功完成验证研究后，被授予 AOAC 性能测试方法 SM 认证，可用于某些食品。
本研究的目的是验证 Soleris 大肠菌群方法在干大麻花（>0.3%Δ9-四氢大麻酚）中的应用。
进行了对比基质研究，用 Soleris 大肠菌群方法和美国食品和药物管理局细菌分析手册固体培养基方法检测自然污染的干大麻花。获得了多批干大麻花，对大肠菌群进行了预筛选，并混合制成四个不同污染水平（4.5 至 1600 CFU/g）的测试材料。每种材料均在三个不同的 Soleris 检测阈值下进行测试，这些阈值由接种 Soleris 小瓶所用的稀释度确定。通过概率检测分析来确定两种方法获得的阳性结果数量是否存在显著差异。
对于所有四个干大麻花材料，在三个 Soleris 测试阈值下，Soleris 和培养平板方法获得的阳性结果数量没有显著差异，这通过概率检测分析在 P<0.05 时确定。
Soleris 大肠菌群检测管是一种检测干大麻花中大肠菌群的准确方法。
Soleris 大肠菌群检测方法为大麻行业 QC 人员提供了一种有效分析干大麻花的方法，可在 18-24 小时内得出结果。
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