Soleris® NF-TVC 方法检测干燥大麻花中需氧、嗜温微生物的验证：AOAC 经过性能测试的方法 SM071203。

Validation of the Soleris® NF-TVC Method for Detection of Aerobic, Mesophilic Microorganisms in Dried Cannabis Flower: AOAC Performance Tested MethodSM 071203.

机构信息

NEOGEN Corp., 620 Lesher Pl., Lansing, MI 48912, USA.

TEQ Analytical Laboratories, 12635 E. Montview Blvd. #175, Aurora, CO 80045, USA.

出版信息

J AOAC Int. 2022 Sep 6;105(5):1418-1422. doi: 10.1093/jaoacint/qsac055.

DOI:10.1093/jaoacint/qsac055

PMID:35575382

Abstract

BACKGROUND

Soleris® Non-Fermenting Total Viable Count (NF-TVC) is a growth-based, automated method for semiquantitative detection of aerobic, mesophilic microorganisms in foods and other consumer products such as nutraceuticals and cosmetics. The method was granted AOAC Performance Tested MethodSM status for select foods after successful completion of a validation study.

OBJECTIVE

The objective of the current study was to validate the Soleris NF-TVC method for use with dried cannabis flower [>0.3% delta 9-tetrahydrocannabinol (THC)].

METHODS

The validation consisted of a comparative matrix study in which naturally contaminated dried cannabis flower was tested with the Soleris NF-TVC method and with the AOAC Official Methods of AnalysisSM 966.23 dilution plating method. Multiple lots of dried cannabis flower were obtained, pre-screened for total aerobic, mesophilic viable count levels, and blended to produce test materials at four different levels of contamination ranging from 1.0 × 103 to 2.2 × 105 CFU/g. Each material was tested at three different Soleris detection threshold levels determined by the dilution used to inoculate the Soleris vials. Probability of detection analysis was performed to determine if differences in the number of positive results obtained with the two methods were significant.

RESULTS

For all four dried cannabis flower materials, at all three Soleris test thresholds, there were no significant differences in performance comparing the Soleris and reference dilution plating methods as determined by probability of detection analysis at P < 0.05.

CONCLUSIONS

It is concluded that the Soleris NF-TVC method is an accurate and effective method for detection of aerobic, mesophilic microorganisms in dried cannabis flower.

HIGHLIGHTS

The Soleris NF-TVC method provides cannabis industry quality control personnel with an effective method for analysis of dried cannabis flower and produces results in 24-48 h.

摘要

背景

Soleris®非发酵总活菌计数（NF-TVC）是一种基于生长的自动化方法，用于半定量检测食品和其他消费品（如营养保健品和化妆品）中的需氧、中温微生物。该方法在成功完成验证研究后，被授予用于特定食品的 AOAC 性能测试方法®地位。

目的

本研究的目的是验证 Soleris NF-TVC 方法在干燥大麻花[>0.3%的 δ 9-四氢大麻酚（THC）]中的应用。

方法

验证包括比较基质研究，其中自然污染的干燥大麻花用 Soleris NF-TVC 方法和 AOAC 官方分析方法®966.23 稀释平板法进行测试。获得了多个批次的干燥大麻花，对其总需氧、中温活菌计数水平进行了预筛选，并混合制成了四个不同污染水平的测试材料，范围从 1.0×103 到 2.2×105 CFU/g。每种材料在三个不同的 Soleris 检测阈值下进行测试，这些阈值由接种 Soleris 小瓶的稀释液确定。进行了概率检测分析，以确定两种方法获得的阳性结果数量差异是否显著。