两种体外受精用介质的密度梯度离心后精子回收率的比较。

Comparative sperm recovery rate after density gradient centrifugation with two media for in vitro fertilization.

机构信息

Materbaby - Reprodução Humana e Genética Maringá Brazil Materbaby - Reprodução Humana e Genética. Maringá, Brazil.

Ingá Materiais Médico Hospitalares LTDA Maringá Brazil Ingá Materiais Médico Hospitalares LTDA, Maringá, Brazil.

出版信息

JBRA Assist Reprod. 2023 Mar 30;27(1):25-28. doi: 10.5935/1518-0557.20220008.

DOI:10.5935/1518-0557.20220008

PMID:35575660

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10065782/

Abstract

OBJECTIVE

To compare the efficacy of two density gradient centrifugation media for retrieving spermatozoa from semen samples by evaluating the total motile sperm count (TMSC) and the percentage recovery.

METHODS

Twenty-two men with different sperm counts participated in the study. The samples were divided into two equal aliquots and processed using the commercial ISolate Sperm Separation Medium (Irvine Scientific, United States) and the GV Gradiente (IngáMed, Brazil). After separation, samples were counted and evaluated for motile sperm recovery.

RESULTS

The mean TMSC in the fresh sample was 19.65±21.08 million/mL. After the ISolate separation the TMSC was 6.71±7.29 million/mL, and for the GV Gradiente it was 6.27±6.82 million/mL. The percentage of motile spermatozoa recovered was 36.47%±21.61 for ISolate and 35.22%±21.24 for the GV Gradiente (p>0.05). The samples from 6 oligospermic patients (27%) were evaluated separately and the TMSC for ISolate was 4.83±2.92 million/mL, and for the GV Gradiente, it was 4.16±3.12 million/mL (p=0.54). When evaluating only normospermic patient samples, the TMSC for ISolate was 9.05±7.29 million/mL, and for the GV Gradiente, it was 8.47±6.79 million/mL (p=0.83).

CONCLUSIONS

There was no statistical difference in retrieving motile sperm using the GV Gradiente and the ISolate Separation Medium.

摘要

目的

通过评估总活动精子计数（TMSC）和回收率百分比，比较两种密度梯度离心介质从精液样本中回收精子的效果。

方法

22 名精子计数不同的男性参与了这项研究。将样本分为两份等量，分别使用商业的 ISolate 精子分离介质（美国 Irvine Scientific）和 GV Gradiente（巴西 IngáMed）进行处理。分离后，对样本进行计数并评估可活动精子的回收率。

结果

新鲜样本的平均 TMSC 为 1965±2108 万/mL。经过 ISolate 分离后，TMSC 为 671±729 万/mL，而 GV Gradiente 则为 627±682 万/mL。ISolate 回收的活动精子百分比为 36.47%±21.61%，GV Gradiente 为 35.22%±21.24%（p>0.05）。对 6 名少精子症患者（27%）的样本进行单独评估，ISolate 的 TMSC 为 483±292 万/mL，GV Gradiente 则为 416±312 万/mL（p=0.54）。仅评估正常精子症患者的样本时，ISolate 的 TMSC 为 905±729 万/mL，GV Gradiente 为 847±679 万/mL（p=0.83）。