Faculty of Veterinary Medicine, Department of Reproduction and Artificial Insemination, Afyon Kocatepe University, Ahmet Necdet Sezer Campus, 03200, Afyonkarahisar, Turkey.
Department of Medicinal and Aromatic Plants, Vocational School of Technical Sciences, Aksaray University, Hacilar Harmanı Street, 12. Boulevard No: 2, 68100, Aksaray, Turkey.
Pol J Vet Sci. 2022 Mar;25(1):5-12. doi: 10.24425/pjvs.2022.140834.
In this study, the cryoprotective effect of different doses of propolis (P) on bull semen, which has solid pharmacological properties thanks to its rich phenolic components, was investigated biochemically and physiologically. Semen samples were collected from Simmental breed bulls via the artificial vagina and pooled. After dividing into five groups, control (C: no additive) and four different P (200, 100, 50, and 25 μg/mL) groups, the final concentration was diluted to 16×106 per straw. Semen samples were equilibrated at 4°C for approximately 4 hours, then placed in French straws and frozen. After thawing, sperm motility and kinetic parameters, DNA integrity by single-cell gel electrophoresis, sperm abnormalities by liquid fixation, and lipid peroxidation levels by the colorimetric method was analyzed by Computer-Assisted Semen Analyzer. P added to the diluent showed no effect on motility and kinetic parameters at P25 and P50 (p>0.05), while P100 and P200 had a negative effect (p⟨0.001). The addition of P (25 and 50) showed a treatment effect on tail abnormality compared to C (p⟨0.05). Especially P50 had a positive effect on tail length, tail DNA, and tail movement, while P100 and P200 caused DNA damage (p⟨0.001). MDA levels increased in all P dose groups compared to C (p⟨0.001). This study has clearly demonstrated that P25 and P50 supplements could be used therapeutically to treat sperm tail abnormalities and prevent DNA damage in post-thawed bull sperm.
在这项研究中，研究人员通过生化和生理学方法研究了不同剂量的蜂胶（P）对公牛精液的保护作用，蜂胶由于其丰富的酚类成分具有坚实的药理学特性。精液样本通过人工阴道从西门塔尔种公牛中收集并混合。将样本分为五组，对照组（C：无添加剂）和四组不同浓度的 P（200、100、50 和 25 μg/mL），最终浓度稀释至每 straw 16×106。精液样本在 4°C 下平衡约 4 小时，然后放入法国 straw 中冷冻。解冻后，通过计算机辅助精液分析仪分析精子活力和运动参数、单细胞凝胶电泳法评估 DNA 完整性、液体固定法评估精子形态异常以及比色法评估脂质过氧化水平。在 P25 和 P50 时，添加到稀释剂中的 P 对活力和运动参数没有影响（p>0.05），而 P100 和 P200 则有负面影响（p⟨0.001）。与 C 相比，添加 P（25 和 50）对尾部异常有治疗作用（p⟨0.05）。特别是 P50 对尾部长度、尾部 DNA 和尾部运动有积极影响，而 P100 和 P200 则导致 DNA 损伤（p⟨0.001）。与 C 相比，所有 P 剂量组的 MDA 水平均升高（p⟨0.001）。这项研究清楚地表明，P25 和 P50 补充剂可以用于治疗公牛精子尾部异常，并防止解冻后精子的 DNA 损伤。
